3ogr

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[[Image:3ogr.jpg|left|200px]]
 
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==Complex structure of beta-galactosidase from Trichoderma reesei with galactose==
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The line below this paragraph, containing "STRUCTURE_3ogr", creates the "Structure Box" on the page.
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<StructureSection load='3ogr' size='340' side='right'caption='[[3ogr]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ogr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OGR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_3ogr| PDB=3ogr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ogr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ogr OCA], [https://pdbe.org/3ogr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ogr RCSB], [https://www.ebi.ac.uk/pdbsum/3ogr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ogr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q70SY0_HYPJE Q70SY0_HYPJE]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have determined the crystal structure of Trichoderma reesei (Hypocrea jecorina) beta-galactosidase (Tr-beta-gal) at a 1.2A resolution and its complex structures with galactose, IPTG and PETG at 1.5, 1.75 and 1.4A resolutions, respectively. Tr-beta-gal is a potential enzyme for lactose hydrolysis in the dairy industry and belongs to family 35 of the glycoside hydrolases (GH-35). The high resolution crystal structures of this six-domain enzyme revealed interesting features about the structure of Tr-beta-gal. We discovered conformational changes in the two loop regions in the active site, implicating a conformational selection-mechanism for the enzyme. In addition, the Glu200, an acid/base catalyst showed two different conformations which undoubtedly affect the pK(a) value of this residue and the catalytic mechanism. The electron density showed extensive glycosylation, suggesting a structure stabilizing role for glycans. The longest glycan showed an electron density that extends to the eighth monosaccharide unit in the extended chain. The Tr-beta-gal structure also showed a well-ordered structure for a unique octaserine motif on the surface loop of the fifth domain.
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===Complex structure of beta-galactosidase from Trichoderma reesei with galactose===
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Crystal structures of Trichoderma reesei beta-galactosidase reveal conformational changes in the active site.,Maksimainen M, Hakulinen N, Kallio JM, Timoharju T, Turunen O, Rouvinen J J Struct Biol. 2011 Apr;174(1):156-63. Epub 2010 Dec 3. PMID:21130883<ref>PMID:21130883</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ogr" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21130883}}, adds the Publication Abstract to the page
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*[[Galactosidase 3D structures|Galactosidase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21130883 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21130883}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3ogr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OGR OCA].
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[[Category: Trichoderma reesei]]
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[[Category: Maksimainen M]]
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==Reference==
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[[Category: Rouvinen J]]
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<ref group="xtra">PMID:21130883</ref><references group="xtra"/>
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[[Category: Beta-galactosidase]]
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[[Category: Hypocrea jecorina]]
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[[Category: Maksimainen, M.]]
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[[Category: Rouvinen, J.]]
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Current revision

Complex structure of beta-galactosidase from Trichoderma reesei with galactose

PDB ID 3ogr

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