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3pfu

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[[Image:3pfu.jpg|left|200px]]
 
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==N-terminal domain of Thiol:disulfide interchange protein DsbD in its reduced form==
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The line below this paragraph, containing "STRUCTURE_3pfu", creates the "Structure Box" on the page.
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<StructureSection load='3pfu' size='340' side='right'caption='[[3pfu]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3pfu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PFU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
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{{STRUCTURE_3pfu| PDB=3pfu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pfu OCA], [https://pdbe.org/3pfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pfu RCSB], [https://www.ebi.ac.uk/pdbsum/3pfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pfu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DSBD_ECOLI DSBD_ECOLI] Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm, thereby maintaining the active site of DsbC, DsbE and DsbG in a reduced state. This transfer involves a cascade of disulfide bond formation and reduction steps.[HAMAP-Rule:MF_00399]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacterial growth and pathogenicity depend on the correct formation of disulfide bonds, a process controlled by the Dsb system in the periplasm of Gram-negative bacteria. Proteins with a thioredoxin fold play a central role in this process. A general feature of thiol-disulfide exchange reactions is the need to avoid a long lived product complex between protein partners. We use a multidisciplinary approach, involving NMR, x-ray crystallography, surface plasmon resonance, mutagenesis, and in vivo experiments, to investigate the interaction between the two soluble domains of the transmembrane reductant conductor DsbD. Our results show oxidation state-dependent affinities between these two domains. These observations have implications for the interactions of the ubiquitous thioredoxin-like proteins with their substrates, provide insight into the key role played by a unique redox partner with an immunoglobulin fold, and are of general importance for oxidative protein-folding pathways in all organisms.
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===N-terminal domain of Thiol:disulfide interchange protein DsbD in its reduced form===
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Oxidation state-dependent protein-protein interactions in disulfide cascades.,Mavridou DA, Saridakis E, Kritsiligkou P, Goddard AD, Stevens JM, Ferguson SJ, Redfield C J Biol Chem. 2011 Jul 15;286(28):24943-56. Epub 2011 May 3. PMID:21543317<ref>PMID:21543317</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3pfu" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3pfu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PFU OCA].
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*[[Thiol:disulfide interchange protein 3D structures|Thiol:disulfide interchange protein 3D structures]]
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[[Category: Escherichia coli]]
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== References ==
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[[Category: Protein-disulfide reductase]]
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<references/>
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[[Category: Ferguson, S J.]]
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__TOC__
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[[Category: Mavridou, D A.I.]]
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</StructureSection>
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[[Category: Redfield, C.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Saridakis, E.]]
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[[Category: Large Structures]]
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[[Category: Ferguson SJ]]
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[[Category: Mavridou DAI]]
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[[Category: Redfield C]]
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[[Category: Saridakis E]]

Current revision

N-terminal domain of Thiol:disulfide interchange protein DsbD in its reduced form

PDB ID 3pfu

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