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3pl1

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[[Image:3pl1.jpg|left|200px]]
 
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==Determination of the crystal structure of the pyrazinamidase from M.tuberculosis : a structure-function analysis for prediction resistance to pyrazinamide.==
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The line below this paragraph, containing "STRUCTURE_3pl1", creates the "Structure Box" on the page.
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<StructureSection load='3pl1' size='340' side='right'caption='[[3pl1]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3pl1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3gbc 3gbc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PL1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
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{{STRUCTURE_3pl1| PDB=3pl1 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pl1 OCA], [https://pdbe.org/3pl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pl1 RCSB], [https://www.ebi.ac.uk/pdbsum/3pl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pl1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q50575_MYCTX Q50575_MYCTX]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pyrazinamidase (PncA) activates the first-line antituberculous drug pyrazinamide into pyrazinoic acid. The crystal structure of the Mycobacterium tuberculosis PncA protein has been determined, showing significant differences in the substrate binding cavity when compared to the pyrazinamidases from Pyrococcus horikoshii and Acinetobacter baumanii. In M. tuberculosis, this region was found to hold a Fe(2+) ion coordinated by one aspartate and three histidines, one of them corresponding to His57 which is replaced by Asp in Mycobacterium bovis, a species naturally resistant to pyrazinamide. The binding cavity also contains a Cys138-Asp8-Lys96 motif evocating a cysteine-based catalytic mechanism. Mutants have been constructed and investigated by kinetic and thermal shift assays, highlighting the importance of protein folding and thermal stability in the pyrazinamidase activity.
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===Determination of the crystal structure of the pyrazinamidase from M.tuberculosis : a structure-function analysis for prediction resistance to pyrazinamide.===
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Crystal Structure of the Pyrazinamidase of Mycobacterium tuberculosis: Insights into Natural and Acquired Resistance to Pyrazinamide.,Petrella S, Gelus-Ziental N, Maudry A, Laurans C, Boudjelloul R, Sougakoff W PLoS One. 2011 Jan 24;6(1):e15785. PMID:21283666<ref>PMID:21283666</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[3pl1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3gbc 3gbc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PL1 OCA].
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<div class="pdbe-citations 3pl1" style="background-color:#fffaf0;"></div>
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[[Category: Mycobacterium tuberculosis]]
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== References ==
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[[Category: Nicotinamidase]]
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<references/>
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[[Category: Mayer, C.]]
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__TOC__
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[[Category: Petrella, S.]]
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</StructureSection>
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[[Category: Sougakoff, W.]]
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[[Category: Large Structures]]
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[[Category: Ziental-Gelus, N.]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Gelus-Ziental N]]
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[[Category: Mayer C]]
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[[Category: Petrella S]]
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[[Category: Sougakoff W]]

Current revision

Determination of the crystal structure of the pyrazinamidase from M.tuberculosis : a structure-function analysis for prediction resistance to pyrazinamide.

PDB ID 3pl1

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