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3puk

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{{STRUCTURE_3puk| PDB=3puk | SCENE= }}
 
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===Re-refinement of the crystal structure of Munc18-3 and Syntaxin4 N-peptide complex===
 
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{{ABSTRACT_PUBMED_21193638}}
 
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==Function==
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==Re-refinement of the crystal structure of Munc18-3 and Syntaxin4 N-peptide complex==
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[[http://www.uniprot.org/uniprot/STXB3_MOUSE STXB3_MOUSE]] Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.<ref>PMID:9045631</ref> [[http://www.uniprot.org/uniprot/STX4_MOUSE STX4_MOUSE]] Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.<ref>PMID:9045631</ref> <ref>PMID:10394363</ref> <ref>PMID:18827011</ref>
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<StructureSection load='3puk' size='340' side='right'caption='[[3puk]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3puk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2pjx 2pjx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PUK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.054&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3puk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3puk OCA], [https://pdbe.org/3puk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3puk RCSB], [https://www.ebi.ac.uk/pdbsum/3puk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3puk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/STXB3_MOUSE STXB3_MOUSE] Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.<ref>PMID:9045631</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Munc18-1 and Syntaxin1 are essential proteins for SNARE-mediated neurotransmission. Munc18-1 participates in synaptic vesicle fusion via dual roles: as a docking/chaperone protein by binding closed Syntaxin1, and as a fusion protein that binds SNARE complexes in a Syntaxin1 N-peptide dependent manner. The two roles are associated with a closed-open Syntaxin1 conformational transition. Here, we show that Syntaxin N-peptide binding to Munc18-1 is not highly selective, suggesting that other parts of the SNARE complex are involved in binding to Munc18-1. We also find that Syntaxin1, with an N peptide and a physically anchored C terminus, binds to Munc18-1 and that this complex can participate in SNARE complex formation. We report a Munc18-1-N-peptide crystal structure that, together with other data, reveals how Munc18-1 might transit from a conformation that binds closed Syntaxin1 to one that may be compatible with binding open Syntaxin1 and SNARE complexes. Our results suggest the possibility that structural transitions occur in both Munc18-1 and Syntaxin1 during their binary interaction. We hypothesize that Munc18-1 domain 3a undergoes a conformational change that may allow coiled-coil interactions with SNARE complexes.
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==About this Structure==
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Possible roles for Munc18-1 domain 3a and Syntaxin1 N-peptide and C-terminal anchor in SNARE complex formation.,Hu SH, Christie MP, Saez NJ, Latham CF, Jarrott R, Lua LH, Collins BM, Martin JL Proc Natl Acad Sci U S A. 2010 Dec 30. PMID:21193638<ref>PMID:21193638</ref>
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[[3puk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2pjx 2pjx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PUK OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:021193638</ref><references group="xtra"/><references/>
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</div>
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[[Category: Lk3 transgenic mice]]
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<div class="pdbe-citations 3puk" style="background-color:#fffaf0;"></div>
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[[Category: Christie, M P.]]
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[[Category: Collins, B M.]]
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==See Also==
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[[Category: Hu, S H.]]
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*[[Syntaxin-binding protein|Syntaxin-binding protein]]
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[[Category: Jarrott, R.]]
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== References ==
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[[Category: Latham, C F.]]
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<references/>
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[[Category: Lua, L H.L.]]
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__TOC__
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[[Category: Martin, J L.]]
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</StructureSection>
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[[Category: Saez, N J.]]
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[[Category: Large Structures]]
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[[Category: Endocytosis-exocytosis complex]]
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[[Category: Mus musculus]]
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[[Category: Membrane trafficking]]
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[[Category: Christie MP]]
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[[Category: Sm protein]]
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[[Category: Collins BM]]
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[[Category: Snare protein]]
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[[Category: Hu S-H]]
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[[Category: Syntaxin]]
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[[Category: Jarrott R]]
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[[Category: Syntaxin binding protein]]
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[[Category: Latham CF]]
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[[Category: Lua LHL]]
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[[Category: Martin JL]]
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[[Category: Saez NJ]]

Current revision

Re-refinement of the crystal structure of Munc18-3 and Syntaxin4 N-peptide complex

PDB ID 3puk

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