This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3t5a

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (17:28, 1 November 2023) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3t5a.png|left|200px]]
 
-
<!--
+
==Crystal structure of N-terminal domain of FAAL28 G330W mutant from Mycobacterium tuberculosis==
-
The line below this paragraph, containing "STRUCTURE_3t5a", creates the "Structure Box" on the page.
+
<StructureSection load='3t5a' size='340' side='right'caption='[[3t5a]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3t5a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T5A FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5a OCA], [https://pdbe.org/3t5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t5a RCSB], [https://www.ebi.ac.uk/pdbsum/3t5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5a ProSAT]</span></td></tr>
-
{{STRUCTURE_3t5a| PDB=3t5a | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/FAA28_MYCTU FAA28_MYCTU] Catalyzes the activation of long-chain fatty acids (C22-24 fatty acids) as acyl-adenylates (acyl-AMP), which are then transferred to the multifunctional polyketide synthase Mas for further chain extension. Involved in the biosynthesis of mycoserates.<ref>PMID:10573420</ref> <ref>PMID:11279114</ref> <ref>PMID:15042094</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Activation of fatty acids as acyl-adenylates by fatty acyl-AMP ligases (FAALs) in Mycobacterium tuberculosis is a variant of a classical theme that involves formation of acyl-CoA (coenzyme A) by fatty acyl-CoA ligases (FACLs). Here, we show that FAALs and FACLs possess similar structural fold and substrate specificity determinants, and the key difference is the absence of a unique insertion sequence in FACL13 structure. A systematic analysis shows a conserved hydrophobic anchorage of the insertion motif across several FAALs. Strikingly, mutagenesis of two phenylalanine residues, which are part of the anchorage, to alanine converts FAAL32 to FACL32. This insertion-based in silico analysis suggests the presence of FAAL homologues in several other non-mycobacterial genomes including eukaryotes. The work presented here establishes an elegant mechanism wherein an insertion sequence drives the functional divergence of FAALs from canonical FACLs.
-
===Crystal structure of N-terminal domain of FAAL28 G330W mutant from Mycobacterium tuberculosis===
+
Molecular Basis of the Functional Divergence of Fatty Acyl-AMP Ligase Biosynthetic Enzymes of Mycobacterium tuberculosis.,Goyal A, Verma P, Anandhakrishnan M, Gokhale RS, Sankaranarayanan R J Mol Biol. 2011 Dec 21. PMID:22206988<ref>PMID:22206988</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_22206988}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 3t5a" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 22206988 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_22206988}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
[[3t5a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5A OCA].
+
[[Category: Mycobacterium tuberculosis H37Rv]]
-
 
+
[[Category: Goyal A]]
-
==Reference==
+
[[Category: Sankaranarayanan R]]
-
<ref group="xtra">PMID:022206988</ref><references group="xtra"/>
+
-
[[Category: Mycobacterium tuberculosis]]
+
-
[[Category: Goyal, A.]]
+
-
[[Category: Sankaranarayanan, R.]]
+
-
[[Category: Acetyl-coa synthetase like fold]]
+
-
[[Category: Amp-binding]]
+
-
[[Category: Ligase]]
+

Current revision

Crystal structure of N-terminal domain of FAAL28 G330W mutant from Mycobacterium tuberculosis

PDB ID 3t5a

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3t5a"
Personal tools