This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5map

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "5map" [edit=sysop:move=sysop])
Current revision (18:35, 1 November 2023) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 5map is ON HOLD
+
==X-ray generated oxyferrous complex of DtpA from Streptomyces lividans==
 +
<StructureSection load='5map' size='340' side='right'caption='[[5map]], [[Resolution|resolution]] 1.49&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[5map]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lividans_TK24 Streptomyces lividans TK24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MAP FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.49&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5map FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5map OCA], [https://pdbe.org/5map PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5map RCSB], [https://www.ebi.ac.uk/pdbsum/5map PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5map ProSAT]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Powerful synergies are available from the combination of multiple methods to study proteins in the crystalline form. Spectroscopies which probe the same region of the crystal from which X-ray crystal structures are determined can give insights into redox, ligand and spin states to complement the information gained from the electron-density maps. The correct assignment of crystal structures to the correct protein redox and ligand states is essential to avoid the misinterpretation of structural data. This is a particular concern for haem proteins, which can occupy a wide range of redox states and are exquisitely sensitive to becoming reduced by solvated electrons generated from interactions of X-rays with water molecules in the crystal. Here, single-crystal spectroscopic fingerprinting has been applied to investigate the laser photoreduction of ferric haem in cytochrome c'. Furthermore, in situ X-ray-driven generation of haem intermediates in crystals of the dye-decolourizing-type peroxidase A (DtpA) from Streptomyces lividans is described.
-
Authors: Moreno Chicano, T., Chaplin, A.K., Worrall, J.A.R., Strange, R.W., Hough, M.A.
+
Photoreduction and validation of haem-ligand intermediate states in protein crystals by in situ single-crystal spectroscopy and diffraction.,Kekilli D, Moreno-Chicano T, Chaplin AK, Horrell S, Dworkowski FSN, Worrall JAR, Strange RW, Hough MA IUCrJ. 2017 Apr 10;4(Pt 3):263-270. doi: 10.1107/S2052252517002159. eCollection, 2017 May 1. PMID:28512573<ref>PMID:28512573</ref>
-
Description: X-ray generated oxyferrous complex of DtpA from Streptomyces lividans
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
-
[[Category: Worrall, J.A.R]]
+
<div class="pdbe-citations 5map" style="background-color:#fffaf0;"></div>
-
[[Category: Moreno Chicano, T]]
+
== References ==
-
[[Category: Chaplin, A.K]]
+
<references/>
-
[[Category: Hough, M.A]]
+
__TOC__
-
[[Category: Strange, R.W]]
+
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Streptomyces lividans TK24]]
 +
[[Category: Chaplin AK]]
 +
[[Category: Hough MA]]
 +
[[Category: Moreno Chicano T]]
 +
[[Category: Strange RW]]
 +
[[Category: Worrall JAR]]

Current revision

X-ray generated oxyferrous complex of DtpA from Streptomyces lividans

PDB ID 5map

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5map"
Personal tools