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3wg3

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{{STRUCTURE_3wg3| PDB=3wg3 | SCENE= }}
 
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===Crystal structure of Agrocybe cylindracea galectin with blood type A antigen tetraose===
 
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{{ABSTRACT_PUBMED_24036446}}
 
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==About this Structure==
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==Crystal structure of Agrocybe cylindracea galectin with blood type A antigen tetraose==
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[[3wg3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WG3 OCA].
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<StructureSection load='3wg3' size='340' side='right'caption='[[3wg3]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3wg3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrocybe_cylindracea Agrocybe cylindracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WG3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WG3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PRD_900132:Blood+group+A+H+type+2+antigen,+beta+anomer'>PRD_900132</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wg3 OCA], [https://pdbe.org/3wg3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wg3 RCSB], [https://www.ebi.ac.uk/pdbsum/3wg3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wg3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ATLE_CYCAE ATLE_CYCAE] Anti-tumor lectin with DNase activity. Inhibits the growth of several tumor cell lines in vitro. Induces lymphocyte infiltration and necrosis of tumor cells in a mouse tumor model. Induces apoptosis in HeLa cells. Binds N-acetylneuraminyl lactose (N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucose) (PubMed:16051274).<ref>PMID:12757412</ref> <ref>PMID:16051274</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A fungal galectin from Agrocybe cylindracea (ACG) exhibits broad binding specificity for beta-galactose-containing glycans. We determined the crystal structures of wild-type ACG and the N46A mutant, with and without glycan ligands. From these structures and a saccharide-binding analysis of the N46A mutant, we revealed that a conformational change of a unique insertion sequence containing Asn46 provides two binding modes for ACG, and thereby confers broad binding specificity. We propose that the unique sequence provides these two distinct glycan-binding modes by an induced-fit mechanism.
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==Reference==
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Conformational change of a unique sequence in a fungal galectin from Agrocybe cylindracea controls glycan ligand-binding specificity.,Kuwabara N, Hu D, Tateno H, Makyio H, Hirabayashi J, Kato R FEBS Lett. 2013 Nov 15;587(22):3620-5. doi: 10.1016/j.febslet.2013.08.046. Epub, 2013 Sep 10. PMID:24036446<ref>PMID:24036446</ref>
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<ref group="xtra">PMID:024036446</ref><references group="xtra"/><references/>
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[[Category: Hirabayashi, J.]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Hu, D.]]
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</div>
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[[Category: Kato, R.]]
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<div class="pdbe-citations 3wg3" style="background-color:#fffaf0;"></div>
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[[Category: Kuwabara, N.]]
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[[Category: Makio, H.]]
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==See Also==
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[[Category: Tateno, H.]]
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*[[Galectin 3D structures|Galectin 3D structures]]
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[[Category: Galactose binding]]
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== References ==
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[[Category: Galectin]]
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<references/>
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[[Category: Sugar binding protein]]
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__TOC__
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</StructureSection>
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[[Category: Agrocybe cylindracea]]
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[[Category: Large Structures]]
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[[Category: Hirabayashi J]]
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[[Category: Hu D]]
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[[Category: Kato R]]
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[[Category: Kuwabara N]]
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[[Category: Makio H]]
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[[Category: Tateno H]]

Current revision

Crystal structure of Agrocybe cylindracea galectin with blood type A antigen tetraose

PDB ID 3wg3

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