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3x00
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the core streptavidin mutant V212 (Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N) complexed with bis iminobiotin long tail (Bis-IMNtail) at 1.3 A resolution== | |
| + | <StructureSection load='3x00' size='340' side='right'caption='[[3x00]], [[Resolution|resolution]] 1.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3x00]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X00 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDN:ETHANE-1,2-DIAMINE'>EDN</scene>, <scene name='pdbligand=ZOF:6-({5-[(2E,3AS,4S,6AR)-2-IMINOHEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL]PENTANOYL}AMINO)HEXANOIC+ACID'>ZOF</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x00 OCA], [https://pdbe.org/3x00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x00 RCSB], [https://www.ebi.ac.uk/pdbsum/3x00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x00 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The streptavidin/biotin interaction has been widely used as a useful tool in research fields. For application to a pre-targeting system, we previously developed a streptavidin mutant that binds to an iminobiotin analog while abolishing affinity for natural biocytin. Here, we design a bivalent iminobiotin analog that shows 1000-fold higher affinity than before, and determine its crystal structure complexed with the mutant protein. | ||
| - | + | Structure-based design and synthesis of a bivalent iminobiotin analog showing strong affinity toward a low immunogenic streptavidin mutant.,Kawato T, Mizohata E, Shimizu Y, Meshizuka T, Yamamoto T, Takasu N, Matsuoka M, Matsumura H, Kodama T, Kanai M, Doi H, Inoue T, Sugiyama A Biosci Biotechnol Biochem. 2015 Jan 3:1-3. PMID:25560769<ref>PMID:25560769</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 3x00" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: Kanai | + | ==See Also== |
| - | [[Category: | + | *[[Avidin 3D structures|Avidin 3D structures]] |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Streptomyces avidinii]] |
| - | [[Category: | + | [[Category: Doi H]] |
| - | [[Category: | + | [[Category: Inoue T]] |
| - | [[Category: | + | [[Category: Kanai M]] |
| + | [[Category: Kawato T]] | ||
| + | [[Category: Kodama T]] | ||
| + | [[Category: Matsumura H]] | ||
| + | [[Category: Matsuoka M]] | ||
| + | [[Category: Meshizuka T]] | ||
| + | [[Category: Mizohata E]] | ||
| + | [[Category: Shimizu Y]] | ||
| + | [[Category: Sugiyama A]] | ||
| + | [[Category: Takasu N]] | ||
| + | [[Category: Yamamoto T]] | ||
Current revision
Crystal structure of the core streptavidin mutant V212 (Y22S/N23D/S27D/S45N/Y83S/R84K/E101D/R103K/E116N) complexed with bis iminobiotin long tail (Bis-IMNtail) at 1.3 A resolution
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Categories: Large Structures | Streptomyces avidinii | Doi H | Inoue T | Kanai M | Kawato T | Kodama T | Matsumura H | Matsuoka M | Meshizuka T | Mizohata E | Shimizu Y | Sugiyama A | Takasu N | Yamamoto T
