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4hz6

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[[Image:4hz6.jpg|left|200px]]
 
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{{STRUCTURE_4hz6| PDB=4hz6 | SCENE= }}
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==crystal structure of BglB==
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<StructureSection load='4hz6' size='340' side='right'caption='[[4hz6]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4hz6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3fiy 3fiy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HZ6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hz6 OCA], [https://pdbe.org/4hz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hz6 RCSB], [https://www.ebi.ac.uk/pdbsum/4hz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hz6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q0GMU3_9BACT Q0GMU3_9BACT]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Beta-glucosidase enzymes (EC 3.2.1-3.2.3) hydrolyze sugars and are implicated in a wide spectrum of biological processes. Recently, we reported that beta-glucosidase has varied kinetic parameters for the natural and synthetic substrates [K.H Nam, S.J. Kim, M.Y. Kim, J.H. Kim, T.S. Yeo, C.M. Lee, H.K Jun, K.Y. Hwang. Crystal structure of engineered beta-glucosidase from a soil metagenome, Proteins 73 (2008) 788-793]. However, an understanding of the kinetic values of beta-glucosidase has not yet enabled the elucidation of its molecular function. Here, we report the X-ray crystal structure of beta-glucosidase with a glucose and cellobiose fragment from uncultured soil metagenome. From the various crystals, we obtained the pre-reaction (native), intermediate (disaccharide cleavage) and post-reaction (glucose binding) states of the active site pocket. These structures provide snapshots of the catalytic processing of beta-glucosidase. In addition, the intermediate state of the crystal structure provides insight into the substrate specificity of beta-glucosidase. These structural studies will facilitate elucidation of the architectural mechanism responsible for the substrate recognition of beta-glucosidase.
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===crystal structure of BglB===
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Structural insights into the substrate recognition properties of beta-glucosidase.,Nam KH, Sung MW, Hwang KY Biochem Biophys Res Commun. 2010 Jan 1;391(1):1131-5. doi:, 10.1016/j.bbrc.2009.12.038. Epub 2009 Dec 11. PMID:20005197<ref>PMID:20005197</ref>
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{{ABSTRACT_PUBMED_20005197}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4hz6" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[4hz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3fiy 3fiy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZ6 OCA].
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*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Uncultured bacterium]]
[[Category: Uncultured bacterium]]
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[[Category: Hwang, K Y.]]
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[[Category: Hwang KY]]
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[[Category: Nam, K H.]]
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[[Category: Nam KH]]
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[[Category: Beta-glucosidase]]
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[[Category: Bgl]]
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[[Category: Bglb]]
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[[Category: Carbohydrate/sugar binding]]
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[[Category: Glucosidase]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Tim barrel]]
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Current revision

crystal structure of BglB

PDB ID 4hz6

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