4k8z
From Proteopedia
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| - | {{STRUCTURE_4k8z| PDB=4k8z | SCENE= }} | ||
| - | ===KOD Polymerase in binary complex with dsDNA=== | ||
| - | {{ABSTRACT_PUBMED_23733496}} | ||
| - | == | + | ==KOD Polymerase in binary complex with dsDNA== |
| - | [[http://www.uniprot.org/uniprot/ | + | <StructureSection load='4k8z' size='340' side='right'caption='[[4k8z]], [[Resolution|resolution]] 2.29Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4k8z]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_kodakarensis_KOD1 Thermococcus kodakarensis KOD1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K8Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.29Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2DA:2,3-DIDEOXYADENOSINE-5-MONOPHOSPHATE'>2DA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k8z OCA], [https://pdbe.org/4k8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k8z RCSB], [https://www.ebi.ac.uk/pdbsum/4k8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k8z ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DPOL_THEKO DPOL_THEKO] Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein. PI-PkoI recognizes 5'-GATTTAGATCCCTGTACC-3' and PI-PkoII recognizes 5'-CAGCTACTACGGTTAC-3'. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Replicate it: Structures of KOD and 9 degrees N DNA polymerases, two enzymes that are widely used to replicate DNA with highly modified nucleotides, were solved at high resolution in complex with primer/template duplex. The data elucidate substrate interaction of the two enzymes and pave the way for further optimisation of the enzymes and substrates. | ||
| - | + | Structures of KOD and 9 degrees N DNA Polymerases Complexed with Primer Template Duplex.,Bergen K, Betz K, Welte W, Diederichs K, Marx A Chembiochem. 2013 Jun 17;14(9):1058-62. doi: 10.1002/cbic.201300175. Epub 2013, Jun 3. PMID:23733496<ref>PMID:23733496</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | < | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4k8z" style="background-color:#fffaf0;"></div> |
| - | [[Category: Thermococcus kodakarensis | + | |
| - | [[Category: Bergen | + | ==See Also== |
| - | [[Category: Betz | + | *[[DNA polymerase 3D structures|DNA polymerase 3D structures]] |
| - | [[Category: Diederichs | + | == References == |
| - | [[Category: Marx | + | <references/> |
| - | [[Category: Welte | + | __TOC__ |
| - | + | </StructureSection> | |
| - | + | [[Category: Large Structures]] | |
| + | [[Category: Thermococcus kodakarensis KOD1]] | ||
| + | [[Category: Bergen K]] | ||
| + | [[Category: Betz K]] | ||
| + | [[Category: Diederichs K]] | ||
| + | [[Category: Marx A]] | ||
| + | [[Category: Welte W]] | ||
Current revision
KOD Polymerase in binary complex with dsDNA
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