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4kmi

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{{STRUCTURE_4kmi| PDB=4kmi | SCENE= }}
 
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===Crystal structure of 4-O-beta-D-mannosyl-D-glucose phosphorylase MGP complexed with PO4===
 
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{{ABSTRACT_PUBMED_23954514}}
 
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==Function==
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==Crystal structure of 4-O-beta-D-mannosyl-D-glucose phosphorylase MGP complexed with PO4==
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[[http://www.uniprot.org/uniprot/MGP_BACFN MGP_BACFN]] Converts 4-O-beta-D-mannopyranosyl-D-glucopyranose (Man-Glc) to mannose 1-phosphate (Man1P) and glucose. Involved in a mannan catabolic pathway which feeds into glycolysis.<ref>PMID:21539815</ref>
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<StructureSection load='4kmi' size='340' side='right'caption='[[4kmi]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4kmi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_fragilis_NCTC_9343 Bacteroides fragilis NCTC 9343]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KMI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KMI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kmi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kmi OCA], [https://pdbe.org/4kmi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kmi RCSB], [https://www.ebi.ac.uk/pdbsum/4kmi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kmi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MGP_BACFN MGP_BACFN] Converts 4-O-beta-D-mannopyranosyl-D-glucopyranose (Man-Glc) to mannose 1-phosphate (Man1P) and glucose. Involved in a mannan catabolic pathway which feeds into glycolysis.<ref>PMID:21539815</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of a novel component of the mannan biodegradation system, 4-O-beta-d-mannosyl-d-glucose phosphorylase (MGP), was determined to a 1.68-A resolution. The structure of the enzyme revealed a unique homohexameric structure, which was formed by using two helices attached to the N-terminus and C-terminus as a tab for sticking between subunits. The structures of MGP complexes with genuine substrates, 4-O-beta-d-mannosyl-d-glucose and phosphate, and the product d-mannose-1-phosphate were also determined. The complex structures revealed that the invariant residue Asp131, which is supposed to be the general acid/base, did not exist close to the glycosidic Glc-O4 atom, which should be protonated in the catalytic reaction. Also, no solvent molecule that might mediate a proton transfer from Asp131 was observed in the substrate complex structure, suggesting that the catalytic mechanism of MGP is different from those of known disaccharide phosphorylases.
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==About this Structure==
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Structure of Novel Enzyme in Mannan Biodegradation Process 4-O-beta-d-Mannosyl-d-Glucose Phosphorylase MGP.,Nakae S, Ito S, Higa M, Senoura T, Wasaki J, Hijikata A, Shionyu M, Ito S, Shirai T J Mol Biol. 2013 Aug 14. pii: S0022-2836(13)00505-6. doi:, 10.1016/j.jmb.2013.08.002. PMID:23954514<ref>PMID:23954514</ref>
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[[4kmi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteroides_fragilis_nctc_9343 Bacteroides fragilis nctc 9343]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KMI OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:023954514</ref><references group="xtra"/><references/>
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</div>
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[[Category: 4-O-beta-D-mannosyl-D-glucose phosphorylase]]
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<div class="pdbe-citations 4kmi" style="background-color:#fffaf0;"></div>
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[[Category: Bacteroides fragilis nctc 9343]]
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== References ==
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[[Category: Higa, M.]]
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<references/>
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[[Category: Hijikata, A.]]
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__TOC__
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[[Category: Ito, S.]]
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</StructureSection>
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[[Category: Nakae, S.]]
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[[Category: Bacteroides fragilis NCTC 9343]]
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[[Category: Senoura, T.]]
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[[Category: Large Structures]]
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[[Category: Shionyu, M.]]
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[[Category: Higa M]]
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[[Category: Shirai, T.]]
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[[Category: Hijikata A]]
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[[Category: Wasaki, J.]]
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[[Category: Ito S]]
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[[Category: 5-bladed beta propeller fold]]
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[[Category: Nakae S]]
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[[Category: Mannan biodegradation]]
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[[Category: Senoura T]]
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[[Category: Phosphorylase]]
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[[Category: Shionyu M]]
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[[Category: Transferase]]
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[[Category: Shirai T]]
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[[Category: Wasaki J]]

Current revision

Crystal structure of 4-O-beta-D-mannosyl-D-glucose phosphorylase MGP complexed with PO4

PDB ID 4kmi

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