This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4mb5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:42, 8 November 2023) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_4mb5| PDB=4mb5 | SCENE= }}
 
-
===Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag5===
 
-
{{ABSTRACT_PUBMED_24598737}}
 
-
==About this Structure==
+
==Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag5==
-
[[4mb5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MB5 OCA].
+
<StructureSection load='4mb5' size='340' side='right'caption='[[4mb5]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[4mb5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moritella_marina Moritella marina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MB5 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.639&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mb5 OCA], [https://pdbe.org/4mb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mb5 RCSB], [https://www.ebi.ac.uk/pdbsum/4mb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mb5 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/B1VBB0_MORMI B1VBB0_MORMI]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, but only a few have been solved. The flexibility of the hinge regions between the domains apparently makes these proteins difficult to crystallize. The analysis of an active-site mutant of MmChi60 in an unliganded form and in complex with the substrates NAG4 and NAG5 revealed significant differences in the substrate-binding site compared with the previously determined complexes of most studied chitinases. A SAXS experiment demonstrated that in addition to the elongated state found in the crystal, the protein can adapt other conformations in solution ranging from fully extended to compact.
-
==Reference==
+
Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.,Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):676-84. doi:, 10.1107/S1399004713032264. Epub 2014 Feb 15. PMID:24598737<ref>PMID:24598737</ref>
-
<ref group="xtra">PMID:024598737</ref><references group="xtra"/><references/>
+
 
-
[[Category: Chitinase]]
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Malecki, P H.]]
+
</div>
-
[[Category: Rypniewski, W.]]
+
<div class="pdbe-citations 4mb5" style="background-color:#fffaf0;"></div>
-
[[Category: Vorgias, C E.]]
+
 
-
[[Category: Alpha/beta-barrel ig-like]]
+
==See Also==
-
[[Category: Chbd]]
+
*[[Chitinase 3D structures|Chitinase 3D structures]]
-
[[Category: Chitin binding domain]]
+
== References ==
-
[[Category: Chitinase]]
+
<references/>
-
[[Category: Hydrolase]]
+
__TOC__
-
[[Category: Hydrolaze]]
+
</StructureSection>
-
[[Category: Immunoglobulin like domain]]
+
[[Category: Large Structures]]
-
[[Category: Ligand binding]]
+
[[Category: Moritella marina]]
-
[[Category: Low activity mutant]]
+
[[Category: Malecki PH]]
-
[[Category: Nag5]]
+
[[Category: Rypniewski W]]
-
[[Category: Tim-barrel]]
+
[[Category: Vorgias CE]]

Current revision

Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag5

PDB ID 4mb5

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4mb5"
Personal tools