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4ng2

From Proteopedia

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Crystal structure of LasR LBD-QslA complex from Pseudomonas aeruginosa

Structural highlights

4ng2 is a 12 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.413Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LASR_PSEAE Transcriptional activator of elastase structural gene (LasB). Binds to the PAI autoinducer.

Publication Abstract from PubMed

The human pathogen Pseudomonas aeruginosa coordinates the expression of virulence factors by using quorum sensing (QS), a signaling cascade triggered by the QS signal molecule and its receptor, a member of the LuxR family of QS transcriptional factors (LasR). The QS threshold and response in P. aeruginosa is defined by a QS LasR-specific antiactivator (QslA), which binds to LasR and prevents it from binding to its target promoter. However, how QslA binds to LasR and regulates its DNA binding activity in QS remains elusive. Here we report the crystal structure of QslA in complex with the N-terminal ligand binding domain of LasR. QsIA exists as a functional dimer to interact with the LasR ligand binding domain. Further analysis shows that QsIA binding occupies the LasR dimerization interface and consequently disrupts LasR dimerization, thereby preventing LasR from binding to its target DNA and disturbing normal QS. Our findings provide a structural model for understanding the QslA-mediated antiactivation mechanism in QS through protein-protein interaction.

QsIA disrupts LasR dimerization in antiactivation of bacterial quorum sensing.,Fan H, Dong Y, Wu D, Bowler MW, Zhang L, Song H Proc Natl Acad Sci U S A. 2013 Dec 6. PMID:24319092^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fan H, Dong Y, Wu D, Bowler MW, Zhang L, Song H. QsIA disrupts LasR dimerization in antiactivation of bacterial quorum sensing. Proc Natl Acad Sci U S A. 2013 Dec 6. PMID:24319092 doi:http://dx.doi.org/10.1073/pnas.1314415110

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ng2"

Categories: Large Structures | Pseudomonas aeruginosa PAO1 | Fan H | Song H | Wu DH

@@ Line 1: / Line 1: @@
 ==Crystal structure of LasR LBD-QslA complex from Pseudomonas aeruginosa==
-<StructureSection load='4ng2' size='340' side='right' caption='[[4ng2]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
+<StructureSection load='4ng2' size='340' side='right'caption='[[4ng2]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ng2]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NG2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NG2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ng2]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NG2 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OHN:N-3-OXO-DODECANOYL-L-HOMOSERINE+LACTONE'>OHN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.413&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lasR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE]), PA1244 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OHN:N-3-OXO-DODECANOYL-L-HOMOSERINE+LACTONE'>OHN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ng2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ng2 OCA], [http://pdbe.org/4ng2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ng2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ng2 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ng2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ng2 OCA], [https://pdbe.org/4ng2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ng2 RCSB], [https://www.ebi.ac.uk/pdbsum/4ng2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ng2 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/LASR_PSEAE LASR_PSEAE]] Transcriptional activator of elastase structural gene (LasB). Binds to the PAI autoinducer.
+[https://www.uniprot.org/uniprot/LASR_PSEAE LASR_PSEAE] Transcriptional activator of elastase structural gene (LasB). Binds to the PAI autoinducer.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 21: @@
 ==See Also==
-*[[Transcriptional activator|Transcriptional activator]]
+*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Pseae]]
+[[Category: Large Structures]]
-[[Category: Fan, H]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-[[Category: Song, H]]
+[[Category: Fan H]]
-[[Category: Wu, D H]]
+[[Category: Song H]]
-[[Category: Antiactivator]]
+[[Category: Wu DH]]
-[[Category: Quorum sensing]]
-[[Category: Transcription regulator]]

4ng2

From Proteopedia

Current revision

Crystal structure of LasR LBD-QslA complex from Pseudomonas aeruginosa

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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