4rsn

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'''Unreleased structure'''
 
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The entry 4rsn is ON HOLD
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==Crystal structure of the E267V mutant of cytochrome P450 BM3==
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<StructureSection load='4rsn' size='340' side='right'caption='[[4rsn]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4rsn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RSN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RSN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.701&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rsn OCA], [https://pdbe.org/4rsn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rsn RCSB], [https://www.ebi.ac.uk/pdbsum/4rsn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rsn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CPXB_PRIM2 CPXB_PRIM2] Functions as a fatty acid monooxygenase (PubMed:3106359, PubMed:1727637, PubMed:16566047, PubMed:7578081, PubMed:11695892, PubMed:14653735, PubMed:16403573, PubMed:18004886, PubMed:17077084, PubMed:17868686, PubMed:18298086, PubMed:18619466, PubMed:18721129, PubMed:19492389, PubMed:20180779, PubMed:21110374, PubMed:21875028). Catalyzes hydroxylation of fatty acids at omega-1, omega-2 and omega-3 positions (PubMed:1727637, PubMed:21875028). Shows activity toward medium and long-chain fatty acids, with optimum chain lengths of 12, 14 and 16 carbons (lauric, myristic, and palmitic acids). Able to metabolize some of these primary metabolites to secondary and tertiary products (PubMed:1727637). Marginal activity towards short chain lengths of 8-10 carbons (PubMed:1727637, PubMed:18619466). Hydroxylates highly branched fatty acids, which play an essential role in membrane fluidity regulation (PubMed:16566047). Also displays a NADPH-dependent reductase activity in the C-terminal domain, which allows electron transfer from NADPH to the heme iron of the cytochrome P450 N-terminal domain (PubMed:3106359, PubMed:1727637, PubMed:16566047, PubMed:7578081, PubMed:11695892, PubMed:14653735, PubMed:16403573, PubMed:18004886, PubMed:17077084, PubMed:17868686, PubMed:18298086, PubMed:18619466, PubMed:18721129, PubMed:19492389, PubMed:20180779, PubMed:21110374, PubMed:21875028). Involved in inactivation of quorum sensing signals of other competing bacteria by oxidazing efficiently acyl homoserine lactones (AHLs), molecules involved in quorum sensing signaling pathways, and their lactonolysis products acyl homoserines (AHs) (PubMed:18020460).<ref>PMID:11695892</ref> <ref>PMID:14653735</ref> <ref>PMID:16403573</ref> <ref>PMID:16566047</ref> <ref>PMID:17077084</ref> <ref>PMID:1727637</ref> <ref>PMID:17868686</ref> <ref>PMID:18004886</ref> <ref>PMID:18020460</ref> <ref>PMID:18298086</ref> <ref>PMID:18619466</ref> <ref>PMID:18721129</ref> <ref>PMID:19492389</ref> <ref>PMID:20180779</ref> <ref>PMID:21110374</ref> <ref>PMID:21875028</ref> <ref>PMID:3106359</ref> <ref>PMID:7578081</ref>
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Authors: Zhou, W.H., Zhang, A.L., Zhang, T., Ren, X.K., Yorke, J.A., Taylor, A., Wong, L.-L.
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==See Also==
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*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
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Description: Crystal structure of the E267V mutant of cytochrome P450 BM3
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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[[Category: Zhang, A.L]]
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__TOC__
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[[Category: Zhang, T]]
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</StructureSection>
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[[Category: Yorke, J.A]]
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[[Category: Large Structures]]
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[[Category: Ren, X.K]]
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[[Category: Priestia megaterium]]
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[[Category: Taylor, A]]
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[[Category: Ren XK]]
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[[Category: Wong, L.-L]]
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[[Category: Taylor A]]
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[[Category: Zhou, W.H]]
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[[Category: Wong L-L]]
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[[Category: Yorke JA]]
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[[Category: Zhang AL]]
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[[Category: Zhang T]]
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[[Category: Zhou WH]]

Current revision

Crystal structure of the E267V mutant of cytochrome P450 BM3

PDB ID 4rsn

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