4u1t
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The crystal structure of holo CalE6, a methionine gamma lyase from Micromonospora echinospora== |
| + | <StructureSection load='4u1t' size='340' side='right'caption='[[4u1t]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4u1t]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U1T FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u1t OCA], [https://pdbe.org/4u1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u1t RCSB], [https://www.ebi.ac.uk/pdbsum/4u1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u1t ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8KNG3_MICEC Q8KNG3_MICEC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CalE6 is a previously uncharacterized protein involved in the biosynthesis of calicheamicins in Micromonospora echinospora. It is a pyridoxal-5'-phosphate-dependent enzyme and exhibits high sequence homology to cystathionine gamma-lyases and cystathionine gamma-synthases. However, it was found to be active towards methionine and to convert this amino acid into alpha-ketobutyrate, ammonium, and methanethiol. The crystal structure of the cofactor-bound holoenzyme was resolved at 2.0 A; it contains two active site residues, Gly105 and Val322, specific for methionine gamma-lyases. Modeling of methionine into the active site allows identification of the active site residues responsible for substrate recognition and catalysis. These findings support that CalE6 is a putative methionine gamma-lyase producing methanethiol as a building block in biosynthesis of calicheamicins. | ||
| - | + | Identification and Characterization of a Methionine gamma-Lyase in the Calicheamicin Biosynthetic Cluster of Micromonospora echinospora.,Song H, Xu R, Guo Z Chembiochem. 2015 Jan 2;16(1):100-9. doi: 10.1002/cbic.201402489. Epub 2014 Nov, 17. PMID:25404066<ref>PMID:25404066</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4u1t" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Methionine gamma-lyase 3D structures|Methionine gamma-lyase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Micromonospora echinospora]] | ||
| + | [[Category: Guo ZH]] | ||
| + | [[Category: Song HG]] | ||
Current revision
The crystal structure of holo CalE6, a methionine gamma lyase from Micromonospora echinospora
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