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4x0r

From Proteopedia

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Current revision

Crystal structure of human MxB stalk domain

Structural highlights

4x0r is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.905Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MX2_HUMAN Interferon-induced dynamin-like GTPase with potent antiviral activity against human immunodeficiency virus type 1 (HIV-1). Acts by targeting the viral capsid and affects the nuclear uptake and/or stability of the HIV-1 replication complex and the subsequent chromosomal integration of the proviral DNA. Exhibits antiviral activity also against simian immunodeficiency virus (SIV-mnd). May play a role in regulating nucleocytoplasmic transport and cell-cycle progression.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Interferon (IFN) is a key component of the innate immune response to exogenous pathogens. Interferon increases the mRNA levels of interferon-stimulated genes (ISGs) in vivo, which is thought to account for its antiviral activity. Recent studies have indicated that human myxovirus resistance protein 2 (Mx2 or MxB), one of these ISGs, contributes to the inhibition of HIV-1 replication by interferon. MxB may bind to HIV-1 relatively late in the post-entry phase, and it leads to a reduced level of integrated viral DNA, thereby restricting HIV-1 infection. The N-terminal 91-aa domain of MxB and the assembly of MxB mediated by the Stalk domain have also been shown to be indispensible for MxB's anti-viral functions, but the mechanism involved has remained elusive. Here, we report the crystal structure (2.9A) of the human MxB Stalk domain. MxB Stalk shows one dimer in the asymmetric unit. Each monomer contains a four-helix bundle. Interestingly, analyses of MxB dimer interfaces show that the majority of residues involved in the interface are not conserved between MxB and MxA, contributing to the building of a more stable MxB dimer. MxA and MxB Stalk domains share 46.7% sequence identity, and the structure of the MxA Stalk domain and the overall structure of MxB Stalk have a similar conformation. Our results indicate that although human Mx proteins share common structural characteristics, their dimerization strategies are unique, contributing to their unique contributions to viral restriction.

Structural insight into the assembly of human anti-HIV dynamin-like protein MxB/Mx2.,Xu B, Kong J, Wang X, Wei W, Xie W, Yu XF Biochem Biophys Res Commun. 2014 Nov 22. pii: S0006-291X(14)02088-9. doi:, 10.1016/j.bbrc.2014.11.058. PMID:25446123^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ King MC, Raposo G, Lemmon MA. Inhibition of nuclear import and cell-cycle progression by mutated forms of the dynamin-like GTPase MxB. Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8957-62. Epub 2004 Jun 7. PMID:15184662 doi:http://dx.doi.org/10.1073/pnas.0403167101
↑ Goujon C, Moncorge O, Bauby H, Doyle T, Ward CC, Schaller T, Hue S, Barclay WS, Schulz R, Malim MH. Human MX2 is an interferon-induced post-entry inhibitor of HIV-1 infection. Nature. 2013 Oct 24;502(7472):559-62. doi: 10.1038/nature12542. Epub 2013 Sep 18. PMID:24048477 doi:http://dx.doi.org/10.1038/nature12542
↑ Liu Z, Pan Q, Ding S, Qian J, Xu F, Zhou J, Cen S, Guo F, Liang C. The interferon-inducible MxB protein inhibits HIV-1 infection. Cell Host Microbe. 2013 Oct 16;14(4):398-410. doi: 10.1016/j.chom.2013.08.015., Epub 2013 Sep 19. PMID:24055605 doi:http://dx.doi.org/10.1016/j.chom.2013.08.015
↑ Kane M, Yadav SS, Bitzegeio J, Kutluay SB, Zang T, Wilson SJ, Schoggins JW, Rice CM, Yamashita M, Hatziioannou T, Bieniasz PD. MX2 is an interferon-induced inhibitor of HIV-1 infection. Nature. 2013 Oct 24;502(7472):563-6. doi: 10.1038/nature12653. Epub 2013 Oct 13. PMID:24121441 doi:http://dx.doi.org/10.1038/nature12653
↑ Xu B, Kong J, Wang X, Wei W, Xie W, Yu XF. Structural insight into the assembly of human anti-HIV dynamin-like protein MxB/Mx2. Biochem Biophys Res Commun. 2014 Nov 22. pii: S0006-291X(14)02088-9. doi:, 10.1016/j.bbrc.2014.11.058. PMID:25446123 doi:http://dx.doi.org/10.1016/j.bbrc.2014.11.058

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4x0r"

Categories: Homo sapiens | Large Structures | Xie W | Yu X-F

@@ Line 1: / Line 1: @@
 ==Crystal structure of human MxB stalk domain==
-<StructureSection load='4x0r' size='340' side='right' caption='[[4x0r]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
+<StructureSection load='4x0r' size='340' side='right'caption='[[4x0r]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4x0r]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X0R FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4x0r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X0R FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x0r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x0r RCSB], [http://www.ebi.ac.uk/pdbsum/4x0r PDBsum]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.905&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x0r OCA], [https://pdbe.org/4x0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x0r RCSB], [https://www.ebi.ac.uk/pdbsum/4x0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x0r ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MX2_HUMAN MX2_HUMAN]] Interferon-induced dynamin-like GTPase with potent antiviral activity against human immunodeficiency virus type 1 (HIV-1). Acts by targeting the viral capsid and affects the nuclear uptake and/or stability of the HIV-1 replication complex and the subsequent chromosomal integration of the proviral DNA. Exhibits antiviral activity also against simian immunodeficiency virus (SIV-mnd). May play a role in regulating nucleocytoplasmic transport and cell-cycle progression.<ref>PMID:15184662</ref> <ref>PMID:24048477</ref> <ref>PMID:24055605</ref> <ref>PMID:24121441</ref>
+[https://www.uniprot.org/uniprot/MX2_HUMAN MX2_HUMAN] Interferon-induced dynamin-like GTPase with potent antiviral activity against human immunodeficiency virus type 1 (HIV-1). Acts by targeting the viral capsid and affects the nuclear uptake and/or stability of the HIV-1 replication complex and the subsequent chromosomal integration of the proviral DNA. Exhibits antiviral activity also against simian immunodeficiency virus (SIV-mnd). May play a role in regulating nucleocytoplasmic transport and cell-cycle progression.<ref>PMID:15184662</ref> <ref>PMID:24048477</ref> <ref>PMID:24055605</ref> <ref>PMID:24121441</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4x0r" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Xie, W]]
+[[Category: Homo sapiens]]
-[[Category: Yu, X F]]
+[[Category: Large Structures]]
-[[Category: Anti-hiv dynamin-like protein]]
+[[Category: Xie W]]
-[[Category: Antiviral protein]]
+[[Category: Yu X-F]]
-[[Category: Dimer]]
-[[Category: Myxovirus resistance protein 2]]
-[[Category: Stalk domain]]

4x0r

From Proteopedia

Current revision

Crystal structure of human MxB stalk domain

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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