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| | ==Crystal structure of EGO-TC== | | ==Crystal structure of EGO-TC== |
| - | <StructureSection load='4xpm' size='340' side='right' caption='[[4xpm]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='4xpm' size='340' side='right'caption='[[4xpm]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xpm]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XPM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XPM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xpm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XPM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XPM FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xpm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xpm OCA], [http://pdbe.org/4xpm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xpm RCSB], [http://www.ebi.ac.uk/pdbsum/4xpm PDBsum]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xpm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xpm OCA], [https://pdbe.org/4xpm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xpm RCSB], [https://www.ebi.ac.uk/pdbsum/4xpm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xpm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MEH1_YEAST MEH1_YEAST]] Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Component of the EGO complex, a complex involved in the regulation of microautophagy.<ref>PMID:15989961</ref> <ref>PMID:16732272</ref> [[http://www.uniprot.org/uniprot/SLM4_YEAST SLM4_YEAST]] Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Component of the EGO complex, a complex involved in the regulation of microautophagy.<ref>PMID:15989961</ref> <ref>PMID:16732272</ref> | + | [https://www.uniprot.org/uniprot/MEH1_YEAST MEH1_YEAST] Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Component of the EGO complex, a complex involved in the regulation of microautophagy.<ref>PMID:15989961</ref> <ref>PMID:16732272</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ding, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Powis, K]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Virgilio, C De]] | + | [[Category: De Virgilio C]] |
| - | [[Category: Zhang, T]] | + | [[Category: Ding J]] |
| - | [[Category: Ego complex]] | + | [[Category: Powis K]] |
| - | [[Category: Ego1]] | + | [[Category: Zhang T]] |
| - | [[Category: Ego2]]
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| - | [[Category: Ego3]]
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| - | [[Category: Protein binding]]
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| - | [[Category: Rapamycin]]
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| - | [[Category: Tor signaling]]
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| Structural highlights
Function
MEH1_YEAST Component of the GSE complex, a GTPase complex required for intracellular sorting of GAP1 out of the endosome. Component of the EGO complex, a complex involved in the regulation of microautophagy.[1] [2]
Publication Abstract from PubMed
The target of rapamycin complex 1 (TORC1) integrates various hormonal and nutrient signals to regulate cell growth, proliferation, and differentiation. Amino acid-dependent activation of TORC1 is mediated via the yeast EGO complex (EGOC) consisting of Gtr1, Gtr2, Ego1, and Ego3. Here, we identify the previously uncharacterized Ycr075w-a/Ego2 protein as an additional EGOC component that is required for the integrity and localization of the heterodimeric Gtr1-Gtr2 GTPases, equivalent to mammalian Rag GTPases. We also report the crystal structure of the Ego1-Ego2-Ego3 ternary complex (EGO-TC) at 2.4 A resolution, in which Ego2 and Ego3 form a heterodimer flanked along one side by Ego1. Structural data also reveal the structural conservation of protein components between the yeast EGO-TC and the human Ragulator, which acts as a GEF for Rag GTPases. Interestingly, however, artificial tethering of Gtr1-Gtr2 to the vacuolar membrane is sufficient to activate TORC1 in response to amino acids even in the absence of the EGO-TC. Our structural and functional data therefore support a model in which the EGO-TC acts as a scaffold for Rag GTPases in TORC1 signaling.Cell Research advance online publication 24 July 2015; doi:10.1038/cr.2015.86.
Crystal structure of the Ego1-Ego2-Ego3 complex and its role in promoting Rag GTPase-dependent TORC1 signaling.,Powis K, Zhang T, Panchaud N, Wang R, De Virgilio C, Ding J Cell Res. 2015 Jul 24. doi: 10.1038/cr.2015.86. PMID:26206314[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Dubouloz F, Deloche O, Wanke V, Cameroni E, De Virgilio C. The TOR and EGO protein complexes orchestrate microautophagy in yeast. Mol Cell. 2005 Jul 1;19(1):15-26. PMID:15989961 doi:10.1016/j.molcel.2005.05.020
- ↑ Gao M, Kaiser CA. A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast. Nat Cell Biol. 2006 Jul;8(7):657-67. Epub 2006 May 28. PMID:16732272 doi:ncb1419
- ↑ Powis K, Zhang T, Panchaud N, Wang R, De Virgilio C, Ding J. Crystal structure of the Ego1-Ego2-Ego3 complex and its role in promoting Rag GTPase-dependent TORC1 signaling. Cell Res. 2015 Jul 24. doi: 10.1038/cr.2015.86. PMID:26206314 doi:http://dx.doi.org/10.1038/cr.2015.86
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