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4y21

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'''Unreleased structure'''
 
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The entry 4y21 is ON HOLD until Paper Publication
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==Crystal Structure of Munc13-1 MUN domain==
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<StructureSection load='4y21' size='340' side='right'caption='[[4y21]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4y21]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y21 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y21 OCA], [https://pdbe.org/4y21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y21 RCSB], [https://www.ebi.ac.uk/pdbsum/4y21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y21 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UN13A_RAT UN13A_RAT] Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Also involved in secretory granule priming in insulin secretion.<ref>PMID:9697857</ref> <ref>PMID:11343654</ref> <ref>PMID:11792326</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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UNC-13-Munc13s have a central function in synaptic-vesicle priming through their MUN domains. However, it is unclear whether this function arises from the ability of the MUN domain to mediate the transition from the Munc18-1-closed syntaxin-1 complex to the SNARE complex in vitro. The crystal structure of the rat Munc13-1 MUN domain now reveals an elongated, arch-shaped architecture formed by alpha-helical bundles, with a highly conserved hydrophobic pocket in the middle. Mutation of two residues (NF) in this pocket abolishes the stimulation caused by the Munc13-1 MUN domain on SNARE-complex assembly and on SNARE-dependent proteoliposome fusion in vitro. Moreover, the same mutation in UNC-13 abrogates synaptic-vesicle priming in Caenorhabditis elegans neuromuscular junctions. These results support the notion that orchestration of syntaxin-1 opening and SNARE-complex assembly underlies the central role of UNC-13-Munc13s in synaptic-vesicle priming.
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Authors: Yang, X.Y., Wang, S., Sheng, Y., Zhang, M., Zou, W.J., Wu, L.J., Kang, L.J., Rizo, J., Zhang, R.G., Xu, T., Ma, C.
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Syntaxin opening by the MUN domain underlies the function of Munc13 in synaptic-vesicle priming.,Yang X, Wang S, Sheng Y, Zhang M, Zou W, Wu L, Kang L, Rizo J, Zhang R, Xu T, Ma C Nat Struct Mol Biol. 2015 Jul;22(7):547-54. doi: 10.1038/nsmb.3038. Epub 2015 Jun, 1. PMID:26030875<ref>PMID:26030875</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Rizo, J]]
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<div class="pdbe-citations 4y21" style="background-color:#fffaf0;"></div>
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[[Category: Yang, X.Y]]
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== References ==
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[[Category: Xu, T]]
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<references/>
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[[Category: Sheng, Y]]
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__TOC__
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[[Category: Wu, L.J]]
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</StructureSection>
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[[Category: Wang, S]]
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[[Category: Large Structures]]
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[[Category: Ma, C]]
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[[Category: Rattus norvegicus]]
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[[Category: Zhang, R.G]]
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[[Category: Kang LJ]]
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[[Category: Zhang, M]]
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[[Category: Ma C]]
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[[Category: Zou, W.J]]
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[[Category: Rizo J]]
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[[Category: Kang, L.J]]
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[[Category: Sheng Y]]
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[[Category: Wang S]]
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[[Category: Wu LJ]]
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[[Category: Xu T]]
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[[Category: Yang XY]]
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[[Category: Zhang M]]
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[[Category: Zhang RG]]
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[[Category: Zou WJ]]

Current revision

Crystal Structure of Munc13-1 MUN domain

PDB ID 4y21

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