4zm3
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4zm3 is ON HOLD Authors: Hirayama, A., Miyanaga, A., Kudo, F., Eguchi, T. Description: Crystal structure of PLP-Dependent 3-Aminobenzoate Synthase ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of PLP-Dependent 3-Aminobenzoate Synthase PctV wild-type== | |
| + | <StructureSection load='4zm3' size='340' side='right'caption='[[4zm3]], [[Resolution|resolution]] 2.27Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4zm3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_pactum Streptomyces pactum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZM3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZM3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zm3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zm3 OCA], [https://pdbe.org/4zm3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zm3 RCSB], [https://www.ebi.ac.uk/pdbsum/4zm3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zm3 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A8R0K5_9ACTN A8R0K5_9ACTN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mutational analysis of the pyridoxal 5'-phosphate (PLP)-dependent enzyme PctV has been carried out to elucidate the multi-step reaction mechanism for the formation of 3-aminobenzoate (3-ABA) from 3-dehydroshikimate (3-DSA). The results revealed that the introduction of a K276R mutation led to the accumulation of a quinonoid intermediate with an absorption maximum of 580 nm following the reaction of pyridoxamine 5'-phosphate (PMP) with 3-DSA. Furthermore, the chemical structure of this intermediate was supported by X-ray crystallographic analysis of the complex formed between the K276R mutant and the quinonoid intermediate. These results clearly show that a quinonoid intermediate is involved in the formation of 3-ABA, and also indicate that the Lys276 residue in the active site of the PctV enzyme plays multiple roles including acid/base catalysis during the dehydration reaction of the quinonoid intermediate, as well as assisting in the PMP formation and aminotransfer reaction. | ||
| - | + | Mechanism-based Trapping of the Quinonoid Intermediate Using the K276R Mutant of PLP-Dependent 3-Aminobenzoate Synthase PctV in the Biosynthesis of Pactamycin.,Hirayama A, Miyanaga A, Kudo F, Eguchi T Chembiochem. 2015 Oct 1. doi: 10.1002/cbic.201500426. PMID:26426567<ref>PMID:26426567</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4zm3" style="background-color:#fffaf0;"></div> |
| - | [[Category: Hirayama | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Miyanaga | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces pactum]] | ||
| + | [[Category: Eguchi T]] | ||
| + | [[Category: Hirayama A]] | ||
| + | [[Category: Kudo F]] | ||
| + | [[Category: Miyanaga A]] | ||
Current revision
Crystal structure of PLP-Dependent 3-Aminobenzoate Synthase PctV wild-type
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