5ms6
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5ms6 is ON HOLD until Paper Publication Authors: Pantoom, S., Vetter, I.R., Wu, Y.W. Description: High-salt structure of RavZ LIR2-fused human LC3B...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==High-salt structure of RavZ LIR2-fused human LC3B== | |
| + | <StructureSection load='5ms6' size='340' side='right'caption='[[5ms6]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ms6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MS6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ms6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ms6 OCA], [https://pdbe.org/5ms6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ms6 RCSB], [https://www.ebi.ac.uk/pdbsum/5ms6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ms6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN] Involved in formation of autophagosomal vacuoles (autophagosomes). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Autophagy is a conserved cellular process involved in the elimination of proteins and organelles. It is also used to combat infection with pathogenic microbes. The intracellular pathogen Legionella pneumophila manipulates autophagy by delivering the effector protein RavZ to deconjugate Atg8/LC3 proteins coupled to phosphatidylethanolamine (PE) on autophagosomal membranes. To understand how RavZ recognizes and deconjugates LC3-PE, we prepared semisynthetic LC3 proteins and elucidated the structures of the RavZ:LC3 interaction. Semisynthetic LC3 proteins allowed the analysis of structure-function relationships. RavZ extracts LC3-PE from the membrane before deconjugation. RavZ initially recognizes the LC3 molecule on membranes via its N-terminal LC3-interacting region (LIR) motif. The RavZ alpha3 helix is involved in extraction of the PE moiety and docking of the acyl chains into the lipid-binding site of RavZ that is related in structure to that of the phospholipid transfer protein Sec14. Thus, Legionella has evolved a novel mechanism to specifically evade host autophagy. | ||
| - | + | Elucidation of the anti-autophagy mechanism of the Legionella effector RavZ using semisynthetic LC3 proteins.,Yang A, Pantoom S, Wu YW Elife. 2017 Apr 11;6. pii: e23905. doi: 10.7554/eLife.23905. PMID:28395732<ref>PMID:28395732</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Pantoom | + | <div class="pdbe-citations 5ms6" style="background-color:#fffaf0;"></div> |
| - | [[Category: Vetter | + | |
| - | [[Category: Wu | + | ==See Also== |
| + | *[[Microtubule-associated protein 3D structures|Microtubule-associated protein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pantoom S]] | ||
| + | [[Category: Vetter IR]] | ||
| + | [[Category: Wu YW]] | ||
Current revision
High-salt structure of RavZ LIR2-fused human LC3B
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