This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5muv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: '''Unreleased structure''' The entry 5muv is ON HOLD until Paper Publication Authors: Sun, Z., El Omari, K., Sun, X., Ilca, S., Kotecha, A., Stuart, D.I., Poranen, M.M., Huiskonen, J.T....)
Current revision (17:50, 8 November 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 5muv is ON HOLD until Paper Publication
+
==Atomic structure fitted into a localized reconstruction of bacteriophage phi6 packaging hexamer P4==
 +
<SX load='5muv' size='340' side='right' viewer='molstar' caption='[[5muv]], [[Resolution|resolution]] 9.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[5muv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MUV FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5muv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5muv OCA], [https://pdbe.org/5muv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5muv RCSB], [https://www.ebi.ac.uk/pdbsum/5muv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5muv ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/P4_BPPH6 P4_BPPH6] P4 is one of the structural proteins of the polyhedral procapsid, which is responsible for genomic replication and transcription. Displays single-stranded RNA-stimulated NTPase activity.<ref>PMID:12239286</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Correct outer protein shell assembly is a prerequisite for virion infectivity in many multi-shelled dsRNA viruses. In the prototypic dsRNA bacteriophage phi6, the assembly reaction is promoted by calcium ions but its biomechanics remain poorly understood. Here, we describe the near-atomic resolution structure of the phi6 double-shelled particle. The outer T=13 shell protein P8 consists of two alpha-helical domains joined by a linker, which allows the trimer to adopt either a closed or an open conformation. The trimers in an open conformation swap domains with each other. Our observations allow us to propose a mechanistic model for calcium concentration regulated outer shell assembly. Furthermore, the structure provides a prime exemplar of bona fide domain-swapping. This leads us to extend the theory of domain-swapping from the level of monomeric subunits and multimers to closed spherical shells, and to hypothesize a mechanism by which closed protein shells may arise in evolution.
-
Authors: Sun, Z., El Omari, K., Sun, X., Ilca, S., Kotecha, A., Stuart, D.I., Poranen, M.M., Huiskonen, J.T.
+
Double-stranded RNA virus outer shell assembly by bona fide domain-swapping.,Sun Z, El Omari K, Sun X, Ilca SL, Kotecha A, Stuart DI, Poranen MM, Huiskonen JT Nat Commun. 2017 Mar 13;8:14814. doi: 10.1038/ncomms14814. PMID:28287099<ref>PMID:28287099</ref>
-
Description: Atomic structure fitted into a localized reconstruction of bacteriophage phi6 packaging hexamer P4
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
-
[[Category: Poranen, M.M]]
+
<div class="pdbe-citations 5muv" style="background-color:#fffaf0;"></div>
-
[[Category: Kotecha, A]]
+
== References ==
-
[[Category: Sun, Z]]
+
<references/>
-
[[Category: El Omari, K]]
+
__TOC__
-
[[Category: Huiskonen, J.T]]
+
</SX>
-
[[Category: Ilca, S]]
+
[[Category: Large Structures]]
-
[[Category: Stuart, D.I]]
+
[[Category: Pseudomonas virus phi6]]
-
[[Category: Sun, X]]
+
[[Category: El Omari K]]
 +
[[Category: Huiskonen JT]]
 +
[[Category: Ilca S]]
 +
[[Category: Kotecha A]]
 +
[[Category: Poranen MM]]
 +
[[Category: Stuart DI]]
 +
[[Category: Sun X]]
 +
[[Category: Sun Z]]

Current revision

Atomic structure fitted into a localized reconstruction of bacteriophage phi6 packaging hexamer P4

5muv, resolution 9.10Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5muv"
Personal tools