1xss

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[[Image:1xss.png|left|200px]]
 
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==Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.==
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The line below this paragraph, containing "STRUCTURE_1xss", creates the "Structure Box" on the page.
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<StructureSection load='1xss' size='340' side='right'caption='[[1xss]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xss]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dipsastraea_favus Dipsastraea favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XSS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYG:(3S)-3-AMINO-3-[(4Z)-1-(CARBOXYMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-IMIDAZOL-2-YL]PROPANOIC+ACID'>DYG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_1xss| PDB=1xss | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xss OCA], [https://pdbe.org/1xss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xss RCSB], [https://www.ebi.ac.uk/pdbsum/1xss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xss ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q53UG7_DIPFA Q53UG7_DIPFA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xs/1xss_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xss ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Kaede is a natural photoconvertible fluorescent protein found in the coral Trachyphyllia geoffroyi. It contains a tripeptide, His 62-Tyr 63-Gly 64, which acts as a green chromophore that is photoconvertible to red following (ultra-) violet irradiation. Here, we report the molecular cloning and crystal structure determination of a new fluorescent protein, KikG, from the coral Favia favus, and its in vitro evolution conferring green-to-red photoconvertibility. Substitution of the His 62-Tyr 63-Gly 64 sequence into the native protein provided only negligible photoconversion. On the basis of the crystal structure, semi-rational mutagenesis of the amino acids surrounding the chromophore was performed, leading to the generation of an efficient highlighter, KikGR. Within mammalian cells, KikGR is more efficiently photoconverted and is several-fold brighter in both the green and red states than Kaede. In addition, KikGR was successfully photoconverted using two-photon excitation microscopy at 760 nm, ensuring optical cell labelling with better spatial discrimination in thick and highly scattering tissues.
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===Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.===
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Semi-rational engineering of a coral fluorescent protein into an efficient highlighter.,Tsutsui H, Karasawa S, Shimizu H, Nukina N, Miyawaki A EMBO Rep. 2005 Mar;6(3):233-8. PMID:15731765<ref>PMID:15731765</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15731765}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1xss" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15731765 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15731765}}
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==About this Structure==
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[[1xss]] is a 2 chain structure of [[Green Fluorescent Protein]] with sequence from [http://en.wikipedia.org/wiki/Favia_favus Favia favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XSS OCA].
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==See Also==
==See Also==
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*[[Green Fluorescent Protein]]
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:015731765</ref><references group="xtra"/>
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__TOC__
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[[Category: Favia favus]]
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</StructureSection>
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[[Category: Karasawa, S.]]
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[[Category: Dipsastraea favus]]
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[[Category: Miyawaki, A.]]
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[[Category: Large Structures]]
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[[Category: Nukina, N.]]
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[[Category: Karasawa S]]
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[[Category: Shimizu, H.]]
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[[Category: Miyawaki A]]
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[[Category: Tsutsui, H.]]
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[[Category: Nukina N]]
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[[Category: Fluorescent protein]]
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[[Category: Shimizu H]]
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[[Category: Luminescent protein]]
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[[Category: Tsutsui H]]

Current revision

Semi-rational engineering of a green-emitting coral fluorescent protein into an efficient highlighter.

PDB ID 1xss

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