|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Structural and spectroscopic characterization of photoconverting fluorescent protein Dendra2== | | ==Structural and spectroscopic characterization of photoconverting fluorescent protein Dendra2== |
| - | <StructureSection load='2vzx' size='340' side='right' caption='[[2vzx]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='2vzx' size='340' side='right'caption='[[2vzx]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2vzx]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dendronephthya_sp._ssal-2002 Dendronephthya sp. ssal-2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VZX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2vzx]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Dendronephthya_sp._SSAL-2002 Dendronephthya sp. SSAL-2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VZX FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5SQ:2-[2-[(1~{S})-1-AZANYL-2-(1~{H}-IMIDAZOL-4-YL)ETHYL]-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXIDANYLIDENE-IMIDAZOL-1-YL]ETHANOIC+ACID'>5SQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vzx OCA], [http://pdbe.org/2vzx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vzx RCSB], [http://www.ebi.ac.uk/pdbsum/2vzx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vzx ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vzx OCA], [https://pdbe.org/2vzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vzx RCSB], [https://www.ebi.ac.uk/pdbsum/2vzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vzx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8T6U0_9CNID Q8T6U0_9CNID] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 29: |
Line 31: |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Green Fluorescent Protein|Green Fluorescent Protein]] | + | *[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Dendronephthya sp. ssal-2002]] | + | [[Category: Dendronephthya sp. SSAL-2002]] |
| - | [[Category: Adam, V]] | + | [[Category: Large Structures]] |
| - | [[Category: Bourgeois, D]] | + | [[Category: Adam V]] |
| - | [[Category: Nienhaus, G U]] | + | [[Category: Bourgeois D]] |
| - | [[Category: Nienhaus, K]] | + | [[Category: Nienhaus GU]] |
| - | [[Category: Dendra2]] | + | [[Category: Nienhaus K]] |
| - | [[Category: Fluorescent protein]]
| + | |
| - | [[Category: Gfp-like protein]]
| + | |
| - | [[Category: Photoactivation]]
| + | |
| Structural highlights
Function
Q8T6U0_9CNID
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Dendra2 is an engineered, monomeric GFP-like protein that belongs to a subclass of fluorescent proteins undergoing irreversible photoconversion from a green- to a red-emitting state upon exposure to purple-blue light. This photoinduced process occurs only in the neutral state of the chromophore and is known to result from backbone cleavage accompanied by an extension of the delocalized pi-electron system. We have measured the X-ray structure of the green species of Dendra2 and performed a comprehensive characterization of the optical absorption and fluorescence properties of the protein in both its green and red forms. The structure, which is very similar to those reported for the closely related proteins EosFP and Kaede, revealed a local structural change involving mainly Arg66 and a water molecule W4, which are part of a charged and hydrogen-bonded cluster of amino acids and water molecules next to the chromophore. Unlike in EosFP and Kaede, Arg66 of Dendra2 does not contribute to negative charge stabilization on the imidazolinone ring by hydrogen bonding to the imidazolinone carbonyl. This structural change may explain the blue shift of the absorption and emission bands, as well as the markedly higher pKs of the hydroxyphenyl moiety of the chromophore, which were determined as 7.1 and 7.5 for the green and red species, respectively. The action spectrum of photoconversion coincides with the absorption band of the neutral species. Consequently, its 20-fold enhancement in Dendra2 at physiological pH accounts for the higher photoconversion yield of this protein as compared to EosFP.
Structural basis of enhanced photoconversion yield in green fluorescent protein-like protein Dendra2.,Adam V, Nienhaus K, Bourgeois D, Nienhaus GU Biochemistry. 2009 Jun 9;48(22):4905-15. PMID:19371086[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Adam V, Nienhaus K, Bourgeois D, Nienhaus GU. Structural basis of enhanced photoconversion yield in green fluorescent protein-like protein Dendra2. Biochemistry. 2009 Jun 9;48(22):4905-15. PMID:19371086 doi:10.1021/bi900383a
|
