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6gp0

From Proteopedia

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'''Unreleased structure'''
 
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The entry 6gp0 is ON HOLD until sometime in the future
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==Structure of mEos4b in the red fluorescent state==
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<StructureSection load='6gp0' size='340' side='right'caption='[[6gp0]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6gp0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GP0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5SQ:2-[2-[(1~{S})-1-AZANYL-2-(1~{H}-IMIDAZOL-4-YL)ETHYL]-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXIDANYLIDENE-IMIDAZOL-1-YL]ETHANOIC+ACID'>5SQ</scene>, <scene name='pdbligand=NFA:PHENYLALANINE+AMIDE'>NFA</scene>, <scene name='pdbligand=RC7:{(2R)-4-(4-HYDROXYBENZYL)-2-[2-(1H-IMIDAZOL-4-YL)ETHYL]-5-OXO-2,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETALDEHYDE'>RC7</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gp0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gp0 OCA], [https://pdbe.org/6gp0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gp0 RCSB], [https://www.ebi.ac.uk/pdbsum/6gp0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gp0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q5S6Z9_LOBHE Q5S6Z9_LOBHE]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort.
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Authors: De Zitter, E., Adam, V., Byrdin, M., Van Meervelt, L., Dedecker, P., Bourgeois, D.
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Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM.,De Zitter E, Thedie D, Monkemoller V, Hugelier S, Beaudouin J, Adam V, Byrdin M, Van Meervelt L, Dedecker P, Bourgeois D Nat Methods. 2019 Jul 8. pii: 10.1038/s41592-019-0462-3. doi:, 10.1038/s41592-019-0462-3. PMID:31285624<ref>PMID:31285624</ref>
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Description: Structure of mEos4b in the red fluorescent state
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Van Meervelt, L]]
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<div class="pdbe-citations 6gp0" style="background-color:#fffaf0;"></div>
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[[Category: Adam, V]]
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[[Category: Byrdin, M]]
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==See Also==
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[[Category: Bourgeois, D]]
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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[[Category: De Zitter, E]]
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== References ==
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[[Category: Dedecker, P]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Lobophyllia hemprichii]]
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[[Category: Adam V]]
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[[Category: Bourgeois D]]
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[[Category: Byrdin M]]
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[[Category: De Zitter E]]
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[[Category: Dedecker P]]
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[[Category: Van Meervelt L]]

Current revision

Structure of mEos4b in the red fluorescent state

PDB ID 6gp0

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