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6rx8
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6rx8 is ON HOLD until Paper Publication Authors: Grandi, E., Poelarends, G.J., Thunnissen, A.M.W.H. Description: EDDS lyase variant D290M/Y320M wit...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==EDDS lyase variant D290M/Y320M with bound fumarate== | |
| + | <StructureSection load='6rx8' size='340' side='right'caption='[[6rx8]], [[Resolution|resolution]] 1.92Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6rx8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chelativorans_sp._BNC1 Chelativorans sp. BNC1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RX8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rx8 OCA], [https://pdbe.org/6rx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rx8 RCSB], [https://www.ebi.ac.uk/pdbsum/6rx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rx8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q11KV9_CHESB Q11KV9_CHESB] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aspartic acid derivatives with branched N -alkyl or N -arylalkyl substituents are valuable precursors to artificial dipeptide sweeteners such as neotame and advantame, which have wide-ranging applications in the food industry. Despite the potential applications of these amino acid precursors to aspartame-based sweeteners, the development of a biocatalyst to synthesize these compounds in a single asymmetric step is an as yet unmet challenge. Herein we report an enantioselective biocatalytic synthesis of various difficult N -substituted aspartic acids including N -(3,3-dimethylbutyl)-L-aspartic acid and N -[3-(3-hydroxy-4-methoxyphenyl)propyl]-L-aspartic acid, precursors to neotame and advantame respectively, using an engineered variant of ethylenediamine- N , N '-disuccinic acid (EDDS) lyase from Chelativorans sp. BNC1. This engineered C-N lyase (mutant D290M/Y320M) displayed a remarkable 1140-fold increase in activity for the selective hydroamination of fumarate compared to that of the wild-type enzyme, which could be rationalized from the analysis of crystal structures. These results open up new opportunities to develop practical multienzymatic processes for the more sustainable and step-economic synthesis of an important class of food additives. | ||
| - | + | Enantioselective Synthesis of Chiral Synthons for Artificial Dipeptide Sweeteners Catalyzed by an Engineered C-N Lyase.,Zhang J, Grandi E, Fu H, Saravanan T, Bothof L, Tepper PG, Thunnissen AWH, Poelarends GJ Angew Chem Int Ed Engl. 2019 Oct 18. doi: 10.1002/anie.201910704. PMID:31625664<ref>PMID:31625664</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6rx8" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Chelativorans sp. BNC1]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Grandi E]] | ||
| + | [[Category: Poelarends GJ]] | ||
| + | [[Category: Thunnissen AMWH]] | ||
Current revision
EDDS lyase variant D290M/Y320M with bound fumarate
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