7t1j
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7t1j is ON HOLD Authors: Pereira, J.H., Liu, A.K., Shih, P.M., Adams, P.D. Description: Crystal structure of RUBISCO from Rhodospirillaceae bacteri...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of RUBISCO from Rhodospirillaceae bacterium BRH_c57== | |
| + | <StructureSection load='7t1j' size='340' side='right'caption='[[7t1j]], [[Resolution|resolution]] 1.96Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7t1j]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodospirillaceae_bacterium_BRH_c57 Rhodospirillaceae bacterium BRH_c57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T1J FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAP:2-CARBOXYARABINITOL-1,5-DIPHOSPHATE'>CAP</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t1j OCA], [https://pdbe.org/7t1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t1j RCSB], [https://www.ebi.ac.uk/pdbsum/7t1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t1j ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0F2R9T6_9PROT A0A0F2R9T6_9PROT] RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.[ARBA:ARBA00003617][HAMAP-Rule:MF_01339] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Oligomerization is a core structural feature that defines the form and function of many proteins. Most proteins form molecular complexes; however, there remains a dearth of diversity-driven structural studies investigating the evolutionary trajectory of these assemblies. Ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) is one such enzyme that adopts multiple assemblies, although the origins and distribution of its different oligomeric states remain cryptic. Here, we retrace the evolution of ancestral and extant form II RuBisCOs, revealing a complex and diverse history of oligomerization. We structurally characterize a newly discovered tetrameric RuBisCO, elucidating how solvent-exposed surfaces can readily adopt new interactions to interconvert or give rise to new oligomeric states. We further use these principles to engineer and demonstrate how changes in oligomerization can be mediated by relatively few mutations. Our findings yield insight into how structural plasticity may give rise to new oligomeric states. | ||
| - | + | Structural plasticity enables evolution and innovation of RuBisCO assemblies.,Liu AK, Pereira JH, Kehl AJ, Rosenberg DJ, Orr DJ, Chu SKS, Banda DM, Hammel M, Adams PD, Siegel JB, Shih PM Sci Adv. 2022 Aug 26;8(34):eadc9440. doi: 10.1126/sciadv.adc9440. Epub 2022 Aug, 26. PMID:36026446<ref>PMID:36026446</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7t1j" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: Liu | + | ==See Also== |
| - | [[Category: Shih | + | *[[RuBisCO 3D structures|RuBisCO 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Rhodospirillaceae bacterium BRH_c57]] | ||
| + | [[Category: Adams PD]] | ||
| + | [[Category: Liu AK]] | ||
| + | [[Category: Pereira JH]] | ||
| + | [[Category: Shih PM]] | ||
Current revision
Crystal structure of RUBISCO from Rhodospirillaceae bacterium BRH_c57
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