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1oas
From Proteopedia
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[[Image:1oas.gif|left|200px]] | [[Image:1oas.gif|left|200px]] | ||
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'''O-ACETYLSERINE SULFHYDRYLASE FROM SALMONELLA TYPHIMURIUM''' | '''O-ACETYLSERINE SULFHYDRYLASE FROM SALMONELLA TYPHIMURIUM''' | ||
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[[Category: Rao, G S.J.]] | [[Category: Rao, G S.J.]] | ||
[[Category: Schnackerz, K D.]] | [[Category: Schnackerz, K D.]] | ||
| - | [[Category: | + | [[Category: Beta replacement enzyme]] |
| - | [[Category: | + | [[Category: Cystein biosynthesis]] |
| - | [[Category: | + | [[Category: Homodimer]] |
| - | [[Category: | + | [[Category: Plp dependent enzyme]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:36:23 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:36, 3 May 2008
O-ACETYLSERINE SULFHYDRYLASE FROM SALMONELLA TYPHIMURIUM
Overview
The last step in cysteine biosynthesis in enteric bacteria is catalyzed by the pyridoxal 5'-phosphate-dependent enzyme O-acetylserine sulfhydrylase. Here we report the crystal structure at 2.2 A resolution of the A-isozyme of O-acetylserine sulfhydrylase isolated from Salmonella typhimurium. O-acetylserine sulfhydrylase shares the same fold with tryptophan synthase-beta from Salmonella typhimurium but the sequence identity level is below 20%. There are some major structural differences: the loops providing the interface to the alpha-subunit in tryptophan synthase-beta and two surface helices of tryptophan synthase-beta are missing in O-acetylserine sulfhydrylase. The hydrophobic channel for indole transport from the alpha to the beta active site of tryptophan synthase-beta is, not unexpectedly, also absent in O-acetylserine sulfhydrylase. The dimer interface, on the other hand, is more or less conserved in the two enzymes. The active site cleft of O-acetylserine sulfhydrylase is wider and therefore more exposed to the solvent. A possible binding site for the substrate O-acetylserine is discussed.
About this Structure
1OAS is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of O-acetylserine sulfhydrylase from Salmonella typhimurium., Burkhard P, Rao GS, Hohenester E, Schnackerz KD, Cook PF, Jansonius JN, J Mol Biol. 1998;283(1):121-33. PMID:9761678 Page seeded by OCA on Sat May 3 03:36:23 2008
