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5wx9
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of AtERF96 with GCC-box== | |
| + | <StructureSection load='5wx9' size='340' side='right'caption='[[5wx9]], [[Resolution|resolution]] 1.76Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5wx9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WX9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wx9 OCA], [https://pdbe.org/5wx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wx9 RCSB], [https://www.ebi.ac.uk/pdbsum/5wx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wx9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ERF96_ARATH ERF96_ARATH] Probably acts as a transcriptional activator. Binds to the GCC-box pathogenesis-related promoter element. May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal alpha-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, might involve in the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal alpha-helix and AP2/ERF domain. | ||
| - | + | Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box.,Chen CY, Lin PH, Chen KH, Cheng YS Plant Mol Biol. 2020 Nov;104(4-5):483-498. doi: 10.1007/s11103-020-01052-5. Epub , 2020 Aug 19. PMID:32813232<ref>PMID:32813232</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5wx9" style="background-color:#fffaf0;"></div> |
| - | [[Category: Chen | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Arabidopsis thaliana]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chen CY]] | ||
| + | [[Category: Cheng YS]] | ||
Current revision
Crystal Structure of AtERF96 with GCC-box
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