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| | <StructureSection load='5wy2' size='340' side='right'caption='[[5wy2]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='5wy2' size='340' side='right'caption='[[5wy2]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5wy2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WY2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WY2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wy2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WY2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WY2 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SNX5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wy2 OCA], [http://pdbe.org/5wy2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wy2 RCSB], [http://www.ebi.ac.uk/pdbsum/5wy2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wy2 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wy2 OCA], [https://pdbe.org/5wy2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wy2 RCSB], [https://www.ebi.ac.uk/pdbsum/5wy2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wy2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SNX5_HUMAN SNX5_HUMAN]] May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.<ref>PMID:18854019</ref> <ref>PMID:21048941</ref> | + | [https://www.uniprot.org/uniprot/SNX5_HUMAN SNX5_HUMAN] May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.<ref>PMID:18854019</ref> <ref>PMID:21048941</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Sorting nexin|Sorting nexin]] | + | *[[Sorting nexin 3D structures|Sorting nexin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Chlamydia trachomatis]] |
| | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jia, D]] | + | [[Category: Jia D]] |
| - | [[Category: Sun, Q]] | + | [[Category: Sun Q]] |
| - | [[Category: Yong, X]] | + | [[Category: Yong X]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Ince]]
| + | |
| - | [[Category: Px domain]]
| + | |
| - | [[Category: Snx5]]
| + | |
| - | [[Category: Transport protein-unknown function complex]]
| + | |
| Structural highlights
Function
SNX5_HUMAN May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.[1] [2]
Publication Abstract from PubMed
The endosomal trafficking pathways are essential for many cellular activities. They are also important targets by many intracellular pathogens. Key regulators of the endosomal trafficking include the retromer complex and sorting nexins (SNXs). Chlamydia trachomatis effector protein IncE directly targets the retromer components SNX5 and SNX6 and suppresses retromer-mediated transport, but the exact mechanism has remained unclear. We present the crystal structure of the PX domain of SNX5 in complex with IncE, showing that IncE binds to a highly conserved hydrophobic groove of SNX5. The unique helical hairpin of SNX5/6 is essential for binding, explaining the specificity of SNX5/6 for IncE. The SNX5/6-IncE interaction is required for cellular localization of IncE and its inhibitory function. Mechanistically, IncE inhibits the association of CI-MPR cargo with retromer-containing endosomal subdomains. Our study provides new insights into the regulation of retromer-mediated transport and illustrates the intricate competition between host and pathogens in controlling cellular trafficking.
Structural and functional insights into sorting nexin 5/6 interaction with bacterial effector IncE.,Sun Q, Yong X, Sun X, Yang F, Dai Z, Gong Y, Zhou L, Zhang X, Niu D, Dai L, Liu JJ, Jia D Signal Transduct Target Ther. 2017 Jun 30;2:17030. doi: 10.1038/sigtrans.2017.30., eCollection 2017. PMID:29263922[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Lim JP, Wang JT, Kerr MC, Teasdale RD, Gleeson PA. A role for SNX5 in the regulation of macropinocytosis. BMC Cell Biol. 2008 Oct 14;9:58. doi: 10.1186/1471-2121-9-58. PMID:18854019 doi:10.1186/1471-2121-9-58
- ↑ Wang JT, Kerr MC, Karunaratne S, Jeanes A, Yap AS, Teasdale RD. The SNX-PX-BAR family in macropinocytosis: the regulation of macropinosome formation by SNX-PX-BAR proteins. PLoS One. 2010 Oct 29;5(10):e13763. doi: 10.1371/journal.pone.0013763. PMID:21048941 doi:10.1371/journal.pone.0013763
- ↑ Sun Q, Yong X, Sun X, Yang F, Dai Z, Gong Y, Zhou L, Zhang X, Niu D, Dai L, Liu JJ, Jia D. Structural and functional insights into sorting nexin 5/6 interaction with bacterial effector IncE. Signal Transduct Target Ther. 2017 Jun 30;2:17030. doi: 10.1038/sigtrans.2017.30., eCollection 2017. PMID:29263922 doi:http://dx.doi.org/10.1038/sigtrans.2017.30
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