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5xu1

From Proteopedia

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Structure of a non-canonical ABC transporter from Streptococcus pneumoniae R6

Structural highlights

5xu1 is a 4 chain structure with sequence from Streptococcus pneumoniae R6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.3Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8DQF8_STRR6

Publication Abstract from PubMed

The spr0693-spr0694-spr0695 operon of Streptococcus pneumoniae encodes a putative ATP-binding cassette (ABC)-type efflux pump involved in the resistance of antibiotics and antimicrobial peptides. Here we report the crystal structures of Spr0694-0695 at 3.3 A and Spr0693 at 3.0 A resolution, revealing a MacAB-like efflux pump. The dimeric Spr0694-0695 adopts a non-canonical fold of ABC transporter, the transmembrane domain of which consists of eight tightly packed transmembrane helices with an insertion of extracellular domain between the first and second helices, whereas Spr0693 forms a nanotube channel docked onto the ABC transporter. Structural analyses combined with ATPase activity and antimicrobial susceptibility assays, enable us to propose a putative substrate-entrance tunnel with a lateral access controlled by a guard helix. Altogether, our findings provide structural insights and putative transport mechanism of a MacAB-like efflux pump in Gram-positive bacteria.

Structure of a MacAB-like efflux pump from Streptococcus pneumoniae.,Yang HB, Hou WT, Cheng MT, Jiang YL, Chen Y, Zhou CZ Nat Commun. 2018 Jan 15;9(1):196. doi: 10.1038/s41467-017-02741-4. PMID:29335499^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yang HB, Hou WT, Cheng MT, Jiang YL, Chen Y, Zhou CZ. Structure of a MacAB-like efflux pump from Streptococcus pneumoniae. Nat Commun. 2018 Jan 15;9(1):196. doi: 10.1038/s41467-017-02741-4. PMID:29335499 doi:http://dx.doi.org/10.1038/s41467-017-02741-4

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xu1"

@@ Line 3: / Line 3: @@
 <StructureSection load='5xu1' size='340' side='right'caption='[[5xu1]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5xu1]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XU1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XU1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5xu1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_R6 Streptococcus pneumoniae R6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XU1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XU1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xu1 OCA], [http://pdbe.org/5xu1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xu1 RCSB], [http://www.ebi.ac.uk/pdbsum/5xu1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xu1 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xu1 OCA], [https://pdbe.org/5xu1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xu1 RCSB], [https://www.ebi.ac.uk/pdbsum/5xu1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xu1 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8DQF8_STRR6 Q8DQF8_STRR6]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Chen, M T]]
+[[Category: Streptococcus pneumoniae R6]]
-[[Category: Chen, Y]]
+[[Category: Chen MT]]
-[[Category: Hou, W T]]
+[[Category: Chen Y]]
-[[Category: Jiang, Y L]]
+[[Category: Hou WT]]
-[[Category: Yang, H B]]
+[[Category: Jiang YL]]
-[[Category: Zhou, C Z]]
+[[Category: Yang HB]]
-[[Category: Abc transporter]]
+[[Category: Zhou CZ]]
-[[Category: Transport protein]]

5xu1

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Current revision

Structure of a non-canonical ABC transporter from Streptococcus pneumoniae R6

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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