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| | ==Crystal structure of Poz1, Tpz1 and Rap1== | | ==Crystal structure of Poz1, Tpz1 and Rap1== |
| - | <StructureSection load='5xxf' size='340' side='right' caption='[[5xxf]], [[Resolution|resolution]] 3.10Å' scene=''> | + | <StructureSection load='5xxf' size='340' side='right'caption='[[5xxf]], [[Resolution|resolution]] 3.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5xxf]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XXF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XXF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5xxf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] and [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XXF FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5xxe|5xxe]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xxf OCA], [http://pdbe.org/5xxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xxf RCSB], [http://www.ebi.ac.uk/pdbsum/5xxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xxf ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xxf OCA], [https://pdbe.org/5xxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xxf RCSB], [https://www.ebi.ac.uk/pdbsum/5xxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xxf ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/POZ1_SCHPO POZ1_SCHPO]] Telomeric DNA-binding protein that negatively regulates telomerase and telomere length.<ref>PMID:18535244</ref> [[http://www.uniprot.org/uniprot/TPZ1_SCHPO TPZ1_SCHPO]] Telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang and involved in telomere length regulation. recruits poz1 and ccq1 to telomeres, regulating telomere length negatively and positivels respectively.<ref>PMID:16303567</ref> <ref>PMID:18535244</ref> | + | [https://www.uniprot.org/uniprot/POZ1_SCHPO POZ1_SCHPO] Telomeric DNA-binding protein that negatively regulates telomerase and telomere length.<ref>PMID:18535244</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chen, H]] | + | [[Category: Large Structures]] |
| - | [[Category: Lei, M]] | + | [[Category: Schizosaccharomyces pombe]] |
| - | [[Category: Wu, J]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Xue, J]] | + | [[Category: Chen H]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Lei M]] |
| - | [[Category: Hub]] | + | [[Category: Wu J]] |
| - | [[Category: Sheterin]] | + | [[Category: Xue J]] |
| - | [[Category: Telomere]]
| + | |
| Structural highlights
Function
POZ1_SCHPO Telomeric DNA-binding protein that negatively regulates telomerase and telomere length.[1]
Publication Abstract from PubMed
Telomeric shelterin complex caps chromosome ends and plays a crucial role in telomere maintenance and protection. In the fission yeast Schizosaccharomyces pombe, shelterin is composed of telomeric single- and double-stranded DNA-binding protein subcomplexes Pot1-Tpz1 and Taz1-Rap1, which are bridged by their interacting protein Poz1. However, the structure of Poz1 and how Poz1 functions as an interaction hub in the shelterin complex remain unclear. Here we report the crystal structure of Poz1 in complex with Poz1-binding motifs of Tpz1 and Rap1. The crystal structure shows that Poz1 employs two different binding surfaces to interact with Tpz1 and Rap1. Unexpectedly, the structure also reveals that Poz1 adopts a dimeric conformation. Mutational analyses suggest that proper interactions between Tpz1, Poz1, and Rap1 in the shelterin core complex are required for telomere length homeostasis and heterochromatin structure maintenance at telomeres. Structural resemblance between Poz1 and the TRFH domains of other shelterin proteins in fission yeast and humans suggests a model for the evolution of shelterin proteins.
Structure of the fission yeast S. pombe telomeric Tpz1-Poz1-Rap1 complex.,Xue J, Chen H, Wu J, Takeuchi M, Inoue H, Liu Y, Sun H, Chen Y, Kanoh J, Lei M Cell Res. 2017 Dec;27(12):1503-1520. doi: 10.1038/cr.2017.145. Epub 2017 Nov 21. PMID:29160296[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Miyoshi T, Kanoh J, Saito M, Ishikawa F. Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length. Science. 2008 Jun 6;320(5881):1341-4. doi: 10.1126/science.1154819. PMID:18535244 doi:http://dx.doi.org/10.1126/science.1154819
- ↑ Xue J, Chen H, Wu J, Takeuchi M, Inoue H, Liu Y, Sun H, Chen Y, Kanoh J, Lei M. Structure of the fission yeast S. pombe telomeric Tpz1-Poz1-Rap1 complex. Cell Res. 2017 Dec;27(12):1503-1520. doi: 10.1038/cr.2017.145. Epub 2017 Nov 21. PMID:29160296 doi:http://dx.doi.org/10.1038/cr.2017.145
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