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5y9h
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5y9h is ON HOLD until Paper Publication Authors: Zeng, L.H., Zhang, L., Wang, P.R., Meng, G. Description: Crystal structure of SafDAA-dsc complex [...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of SafDAA-dsc complex== | |
| + | <StructureSection load='5y9h' size='340' side='right'caption='[[5y9h]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5y9h]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium] and [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y9H FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y9h OCA], [https://pdbe.org/5y9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y9h RCSB], [https://www.ebi.ac.uk/pdbsum/5y9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y9h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q7CR56_SALTY Q7CR56_SALTY] [https://www.uniprot.org/uniprot/Q8ZRK4_SALTY Q8ZRK4_SALTY] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pili are critical in host recognition, colonization and biofilm formation during bacterial infection. Here, we report the crystal structures of SafD-dsc and SafD-SafA-SafA (SafDAA-dsc) in Saf pili. Cell adherence assays show that SafD and SafA are both required for host recognition, suggesting a poly-adhesive mechanism for Saf pili. Moreover, the SafDAA-dsc structure, as well as SAXS characterization, reveals an unexpected inter-molecular oligomerization, prompting the investigation of Saf-driven self-association in biofilm formation. The bead/cell aggregation and biofilm formation assays are used to demonstrate the novel function of Saf pili. Structure-based mutants targeting the inter-molecular hydrogen bonds and complementary architecture/surfaces in SafDAA-dsc dimers significantly impaired the Saf self-association activity and biofilm formation. In summary, our results identify two novel functions of Saf pili: the poly-adhesive and self-associating activities. More importantly, Saf-Saf structures and functional characterizations help to define a pili-mediated inter-cellular oligomerizaiton mechanism for bacterial aggregation, colonization and ultimate biofilm formation. | ||
| - | + | Structural basis of host recognition and biofilm formation by Salmonella Saf pili.,Zeng L, Zhang L, Wang P, Meng G Elife. 2017 Nov 10;6. doi: 10.7554/eLife.28619. PMID:29125121<ref>PMID:29125121</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5y9h" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium]] | ||
| + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]] | ||
| + | [[Category: Meng G]] | ||
| + | [[Category: Wang PR]] | ||
| + | [[Category: Zeng LH]] | ||
| + | [[Category: Zhang L]] | ||
Current revision
Crystal structure of SafDAA-dsc complex
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