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5zul

From Proteopedia

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Small heat shock protein from Mycobacterium marinum M : Form-3

Structural highlights

5zul is a 6 chain structure with sequence from Mycobacterium marinum M. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.75Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

B2HF11_MYCMM

Publication Abstract from PubMed

Small heat shock proteins (sHSPs) are ATP-independent molecular chaperones present ubiquitously in all kingdoms of life. Their low molecular weight subunits associate to form higher order structures. Under conditions of stress, sHSPs prevent aggregation of substrate proteins by undergoing rapid changes in their conformation or stoichiometry. Polydispersity and dynamic nature of these proteins have made structural investigations through crystallography a daunting task. In pathogens like Mycobacteria, sHSPs are immuno-dominant antigens, enabling survival of the pathogen within the host and contributing to disease persistence. We characterized sHSPs from Mycobacterium marinum M and determined the crystal structure of one of these. The protein crystallized in three different conditions as dodecamers, with dimers arranged in a tetrahedral fashion to form a closed cage-like architecture. Interestingly, we found a pentapeptide bound to the dodecamers revealing one of the modes of sHSP-substrate interaction. Further, we have observed that ATP inhibits the chaperoning activity of the protein.

Dodecameric structure of a small heat shock protein from Mycobacterium marinum M.,Bhandari S, Biswas S, Chaudhary A, Dutta S, Suguna K Proteins. 2019 Jan 11. doi: 10.1002/prot.25657. PMID:30632633^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bhandari S, Biswas S, Chaudhary A, Dutta S, Suguna K. Dodecameric structure of a small heat shock protein from Mycobacterium marinum M. Proteins. 2019 Jan 11. doi: 10.1002/prot.25657. PMID:30632633 doi:http://dx.doi.org/10.1002/prot.25657

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5zul"

Categories: Large Structures | Mycobacterium marinum M | Bhandari S | Suguna K

@@ Line 3: / Line 3: @@
 <StructureSection load='5zul' size='340' side='right'caption='[[5zul]], [[Resolution|resolution]] 3.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5zul]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycmm Mycmm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZUL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5zul]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_marinum_M Mycobacterium marinum M]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZUL FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MMAR_2991 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216594 MYCMM])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.75&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zul OCA], [http://pdbe.org/5zul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zul RCSB], [http://www.ebi.ac.uk/pdbsum/5zul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zul ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zul OCA], [https://pdbe.org/5zul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zul RCSB], [https://www.ebi.ac.uk/pdbsum/5zul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zul ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/B2HF11_MYCMM B2HF11_MYCMM]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 18: @@
 </div>
 <div class="pdbe-citations 5zul" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Heat Shock Protein structures|Heat Shock Protein structures]]
 == References ==
 <references/>
@@ Line 21: / Line 26: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Mycmm]]
+[[Category: Mycobacterium marinum M]]
-[[Category: Bhandari, S]]
+[[Category: Bhandari S]]
-[[Category: Suguna, K]]
+[[Category: Suguna K]]
-[[Category: Chaperone]]
-[[Category: Oligomer]]
-[[Category: Polydispersity]]
-[[Category: Shsp]]

5zul

From Proteopedia

Current revision

Small heat shock protein from Mycobacterium marinum M : Form-3

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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