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| <StructureSection load='5zvj' size='340' side='right'caption='[[5zvj]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='5zvj' size='340' side='right'caption='[[5zvj]], [[Resolution|resolution]] 2.70Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[5zvj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5xdy 5xdy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZVJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZVJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zvj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5xdy 5xdy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZVJ FirstGlance]. <br> |
- | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
- | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> |
- | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">htrA, SAMEA2682835_04373 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zvj OCA], [https://pdbe.org/5zvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zvj RCSB], [https://www.ebi.ac.uk/pdbsum/5zvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zvj ProSAT]</span></td></tr> |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zvj OCA], [http://pdbe.org/5zvj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zvj RCSB], [http://www.ebi.ac.uk/pdbsum/5zvj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zvj ProSAT]</span></td></tr> | + | |
| </table> | | </table> |
| + | == Function == |
| + | [https://www.uniprot.org/uniprot/HTRA1_MYCTU HTRA1_MYCTU] Essential protein that may act as a regulatory protease that is conditionally activated upon appropriate environmental triggers.<ref>PMID:30511675</ref> |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
- | [[Category: Biswal, B K]] | + | [[Category: Mycobacterium tuberculosis]] |
- | [[Category: Khundrakpam, H S]] | + | [[Category: Biswal BK]] |
- | [[Category: Kumar, D]] | + | [[Category: Khundrakpam HS]] |
- | [[Category: Yadav, S]] | + | [[Category: Kumar D]] |
- | [[Category: Htra]] | + | [[Category: Yadav S]] |
- | [[Category: Hydrolase]]
| + | |
- | [[Category: Mycobacterium]]
| + | |
- | [[Category: Pdz]]
| + | |
- | [[Category: Protease]]
| + | |
| Structural highlights
Function
HTRA1_MYCTU Essential protein that may act as a regulatory protease that is conditionally activated upon appropriate environmental triggers.[1]
Publication Abstract from PubMed
High-temperature requirement A (HtrA) proteins, which are members of the heat-shock-induced serine protease family, are involved in extracytoplasmic protein quality control and bacterial survival strategies under stress conditions, and are associated with the virulence of several pathogens; they are therefore major drug targets. Mycobacterium tuberculosis possesses three putative HtrAs: HtrA1 (Rv1223), HtrA2 (Rv0983) and HtrA3 (Rv0125). Each has a cytoplasmic region, a transmembrane helix and a periplasmic region. Here, the crystal structure of the periplasmic region consisting of a protease domain (PD) and a PDZ domain from an M. tuberculosis HtrA1 mutant (mHtrA1(S387A)) is reported at 2.7 A resolution. Although the mHtrA1(S387A) PD shows structural features similar to those of other HtrAs, its loops, particularly L3 and LA, display different conformations. Loop L3 communicates between the PDs of the trimer and the PDZ domains and undergoes a transition from an active to an inactive conformation, as reported for an equivalent HtrA (DegS). Loop LA, which is responsible for higher oligomer formation owing to its length (50 amino acids) in DegP, is very short in mHtrA1(S387A) (five amino acids), as in mHtrA2 (also five amino acids), and therefore lacks essential interactions for the formation of higher oligomers. Notably, a well ordered loop known as the insertion clamp in the PDZ domain interacts with the protease domain of the adjacent molecule, which possibly aids in the stabilization of a trimeric functional unit of this enzyme. The three-dimensional structure of mHtrA1(S387A) presented here will be useful in the design of enzyme-specific antituberculosis inhibitors.
The crystal structure of an essential high-temperature requirement protein HtrA1 (Rv1223) from Mycobacterium tuberculosis reveals its unique features.,Singh KH, Yadav S, Kumar D, Biswal BK Acta Crystallogr D Struct Biol. 2018 Sep 1;74(Pt 9):906-921. doi:, 10.1107/S205979831800952X. Epub 2018 Sep 5. PMID:30198900[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gupta AK, Behera D, Gopal B. The crystal structure of Mycobacterium tuberculosis high-temperature requirement A protein reveals an autoregulatory mechanism. Acta Crystallogr F Struct Biol Commun. 2018 Dec 1;74(Pt 12):803-809. doi:, 10.1107/S2053230X18016217. Epub 2018 Nov 29. PMID:30511675 doi:http://dx.doi.org/10.1107/S2053230X18016217
- ↑ Singh KH, Yadav S, Kumar D, Biswal BK. The crystal structure of an essential high-temperature requirement protein HtrA1 (Rv1223) from Mycobacterium tuberculosis reveals its unique features. Acta Crystallogr D Struct Biol. 2018 Sep 1;74(Pt 9):906-921. doi:, 10.1107/S205979831800952X. Epub 2018 Sep 5. PMID:30198900 doi:http://dx.doi.org/10.1107/S205979831800952X
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