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6a02

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Salmonella Typhi YfdX in the F222 space group at 1.4 A resolution

Structural highlights

6a02 is a 1 chain structure with sequence from Salmonella enterica subsp. enterica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.4Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A0D6IF67_SALTM

Publication Abstract from PubMed

YfdX is a prokaryotic protein encoded by several pathogenic bacteria including Salmonella enterica serovar Typhi, which causes one of the most fatal infectious diseases, typhoid fever. YfdX is a product of the yfdXWUVE operon and is known to be under the control of EvgA, a regulator protein controlling the expression of several proteins involved in response to environmental stress, in Escherichia coli. Nevertheless, unlike other proteins encoded by the same operon, the structural and physiological aspects of YfdX have been poorly characterized. Here, we identified a previously unknown pH-dependent stoichiometric conversion of S. Typhi YfdX between dimeric and tetrameric states; this conversion was further analyzed via determining its structure by X-ray crystallography at high resolution and by small-angle X-ray scattering in a solution state and via structure-based mutant studies. Biologically, YfdX was proven to be critically involved in Salmonella susceptibility to two beta-lactam antibiotics, penicillin G and carbenicillin, as bacterial growth significantly impaired by its deficiency upon treatment with each of the two antibiotics was recovered by chromosomal complementation. Furthermore, by using Galleria mellonella larvae as an in vivo model of Salmonella infection, we demonstrated that Salmonella virulence was remarkably enhanced by YfdX deficiency, which was complemented by a transient expression of the wild-type or dimeric mutant but not by that of the monomeric mutant. The present study work provides direct evidence regarding the participation of YfdX in Salmonella antibiotic susceptibility and in the modulation of bacterial virulence, providing a new insight into this pathogen's strategies for survival and growth.

Structural and Physiological Exploration of Salmonella Typhi YfdX Uncovers Its Dual Function in Bacterial Antibiotic Stress and Virulence.,Lee HS, Lee S, Kim JS, Lee HR, Shin HC, Lee MS, Jin KS, Kim CH, Ku B, Ryu CM, Kim SJ Front Microbiol. 2019 Jan 14;9:3329. doi: 10.3389/fmicb.2018.03329. eCollection, 2018. PMID:30692978^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lee HS, Lee S, Kim JS, Lee HR, Shin HC, Lee MS, Jin KS, Kim CH, Ku B, Ryu CM, Kim SJ. Structural and Physiological Exploration of Salmonella Typhi YfdX Uncovers Its Dual Function in Bacterial Antibiotic Stress and Virulence. Front Microbiol. 2019 Jan 14;9:3329. doi: 10.3389/fmicb.2018.03329. eCollection, 2018. PMID:30692978 doi:http://dx.doi.org/10.3389/fmicb.2018.03329

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6a02"

Categories: Large Structures | Salmonella enterica subsp. enterica | Kim SJ | Ku B | Lee HS

@@ Line 3: / Line 3: @@
 <StructureSection load='6a02' size='340' side='right'caption='[[6a02]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6a02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salet Salet]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A02 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6a02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica Salmonella enterica subsp. enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A02 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">yfdX, CVR97_27555, SE14_03224 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=59201 SALET])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a02 OCA], [https://pdbe.org/6a02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a02 RCSB], [https://www.ebi.ac.uk/pdbsum/6a02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a02 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0D6IF67_SALTM A0A0D6IF67_SALTM]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 27: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Salet]]
+[[Category: Salmonella enterica subsp. enterica]]
-[[Category: Kim, S J]]
+[[Category: Kim SJ]]
-[[Category: Ku, B]]
+[[Category: Ku B]]
-[[Category: Lee, H S]]
+[[Category: Lee HS]]
-[[Category: Salmonella]]
-[[Category: Sty3178]]
-[[Category: Unknown function]]
-[[Category: Yfdx]]

6a02

From Proteopedia

Current revision

Salmonella Typhi YfdX in the F222 space group at 1.4 A resolution

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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