6a6h
From Proteopedia
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(New page: '''Unreleased structure''' The entry 6a6h is ON HOLD Authors: Ning, S., Wang, Z.B. Description: Crystal Structure of Swine Major Histocompatibility Complex Class I SLA-2*040202 For 2.3...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of Swine Major Histocompatibility Complex Class I SLA-2*040202 For 2.3 Angstrom== | |
| + | <StructureSection load='6a6h' size='340' side='right'caption='[[6a6h]], [[Resolution|resolution]] 2.31Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6a6h]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Foot-and-mouth_disease_virus_A Foot-and-mouth disease virus A] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A6H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A6H FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a6h OCA], [https://pdbe.org/6a6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a6h RCSB], [https://www.ebi.ac.uk/pdbsum/6a6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a6h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8MHU4_PIG Q8MHU4_PIG] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Presentation of viral epitopes by swine MHC I (termed leukocyte antigen class I, SLA I) to cytotoxic T lymphocytes (CTLs) is crucial for swine immunity. The SLA-2 structure, however, remains largely unknown. To illustrate the structural basis of swine CTL epitope presentation, the crystal structure of SLA-2*04:02:02 complexed with one peptide, derived from foot-and-mouth disease virus (FMDV), was analyzed in this study. SLA-2*04:02:02 and swine beta2-microglobulin (sbeta2m) were refolded in vitro in the presence of peptides. X-ray diffraction data of SLA-2*04:02:02-peptide-sbeta2m (referred to as p/SLA-2*04:02:02) were collected. The diffraction dataset was 2.3â¯A in resolution and the space group was P3(2)21. Relevant data included aâ¯=â¯101.8â¯A, bâ¯=â¯101.8â¯A, câ¯=â¯73.455â¯A,alphaâ¯=â¯90.00 degrees , betaâ¯=â¯90.00 degrees , gammaâ¯=â¯120.00 degrees . The structure of p/SLA-2*04:02:02 was analyzed. The results revealed that Glu24, Met68, Gly76, and Gln173 in PBG of SLA-2*04:02:02 are different from other MHC I. Furthermore, Asn63 is different from other SLA I. Gln57, Met174 and Gln180 in PBG of SLA I are different from other species' MHC I. All of these features are different from known mammalian peptide-MHC class I complexes (referred to as p/MHC I). In addition, P4-His, P6-Val, and P8-Pro in the peptide were exposed, and these residues as epitopes can be presented by SLA-2*04:02:02. This study not only provides a structural basis for peptide presentation by SLA-2, but also screens one potential FMDV CTL epitope. The results may be of interest in future vaccine development. | ||
| - | + | Crystallization of SLA-2*04:02:02 complexed with a CTL epitope derived from FMDV.,Ning S, Wang ZB, Qi P, Xiao J, Wang XJ Res Vet Sci. 2020 Feb;128:90-98. doi: 10.1016/j.rvsc.2019.11.002. Epub 2019 Nov , 7. PMID:31760318<ref>PMID:31760318</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6a6h" style="background-color:#fffaf0;"></div> |
| - | [[Category: Ning | + | |
| + | ==See Also== | ||
| + | *[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]] | ||
| + | *[[MHC 3D structures|MHC 3D structures]] | ||
| + | *[[MHC I 3D structures|MHC I 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Foot-and-mouth disease virus A]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sus scrofa]] | ||
| + | [[Category: Ning S]] | ||
| + | [[Category: Wang ZB]] | ||
Current revision
Crystal Structure of Swine Major Histocompatibility Complex Class I SLA-2*040202 For 2.3 Angstrom
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