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6akl

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Crystal structure of Striatin3 in complex with SIKE1 Coiled-coil domain

Structural highlights

6akl is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.75Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SIKE1_HUMAN Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.^[1]

Publication Abstract from PubMed

Striatin-interacting phosphatases and kinases (STRIPAKs) are evolutionarily conserved supramolecular complexes, which have been implicated in the Hippo signaling pathway. Yet the topological structure and dynamic assembly of STRIPAK complexes remain elusive. Here, we report the overall architecture and substructures of a Hippo kinase-containing STRIPAK complex. PP2Aa/c-bound STRN3 directly contacts the Hippo kinase MST2 and also controls the loading of MST2 via two "arms" in a phosphorylation-dependent manner, one arm being STRIP1 and the other SIKE1-SLMAP. A decreased cell density triggered the dissociation of the STRIP1 arm from STRIPAK, reflecting the dynamic assembly of the complex upon sensing upstream signals. Crystallographic studies defined at atomic resolution the interface between STRN3 and SIKE1, and that between SIKE1 and SLMAP. Disrupting the complex assembly abrogated the regulatory effect of STRIPAK towards Hippo signaling. Collectively, our study revealed a "two-arm" assembly of STRIPAK with context-dependent dynamics, offering a framework for further studies on Hippo signaling and biological processes involving MST kinases.

Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling.,Tang Y, Chen M, Zhou L, Ma J, Li Y, Zhang H, Shi Z, Xu Q, Zhang X, Gao Z, Zhao Y, Cheng Y, Jiao S, Zhou Z Cell Discov. 2019 Jan 8;5:3. doi: 10.1038/s41421-018-0077-3. eCollection 2019. PMID:30622739^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Huang J, Liu T, Xu LG, Chen D, Zhai Z, Shu HB. SIKE is an IKK epsilon/TBK1-associated suppressor of TLR3- and virus-triggered IRF-3 activation pathways. EMBO J. 2005 Dec 7;24(23):4018-28. doi: 10.1038/sj.emboj.7600863. Epub 2005 Nov, 10. PMID:16281057 doi:http://dx.doi.org/10.1038/sj.emboj.7600863
↑ Tang Y, Chen M, Zhou L, Ma J, Li Y, Zhang H, Shi Z, Xu Q, Zhang X, Gao Z, Zhao Y, Cheng Y, Jiao S, Zhou Z. Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling. Cell Discov. 2019 Jan 8;5:3. doi: 10.1038/s41421-018-0077-3. eCollection 2019. PMID:30622739 doi:http://dx.doi.org/10.1038/s41421-018-0077-3

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6akl"

Categories: Homo sapiens | Large Structures | Chen M | Zhou L | Zhou ZC

@@ Line 1: / Line 1: @@
 ==Crystal structure of Striatin3 in complex with SIKE1 Coiled-coil domain==
-<StructureSection load='6akl' size='340' side='right' caption='[[6akl]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
+<StructureSection load='6akl' size='340' side='right'caption='[[6akl]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6akl]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AKL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6akl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AKL FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SIKE1, SIKE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6akl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akl OCA], [http://pdbe.org/6akl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6akl RCSB], [http://www.ebi.ac.uk/pdbsum/6akl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6akl ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6akl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akl OCA], [https://pdbe.org/6akl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6akl RCSB], [https://www.ebi.ac.uk/pdbsum/6akl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6akl ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SIKE1_HUMAN SIKE1_HUMAN]] Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.<ref>PMID:16281057</ref>  [[http://www.uniprot.org/uniprot/STRN3_HUMAN STRN3_HUMAN]] Binds calmodulin in a calcium dependent manner. May function as scaffolding or signaling protein.
+[https://www.uniprot.org/uniprot/SIKE1_HUMAN SIKE1_HUMAN] Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.<ref>PMID:16281057</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Chen, M]]
+[[Category: Large Structures]]
-[[Category: Zhou, L]]
+[[Category: Chen M]]
-[[Category: Zhou, Z C]]
+[[Category: Zhou L]]
-[[Category: Coiled-coil domain]]
+[[Category: Zhou ZC]]
-[[Category: Heterotrimer]]
-[[Category: Protein binding]]

6akl

From Proteopedia

Current revision

Crystal structure of Striatin3 in complex with SIKE1 Coiled-coil domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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