6igb

Publication Abstract from PubMed

Biofilm formation is a critical determinant in the pathopoiesis of Pseudomonas aeruginosa It could significantly increase bacterial resistance to drugs and host defense. Thus, inhibition of biofilm matrix production could be regarded as a promising attempt to prevent colonization of P. aeruginosa and the subsequent infection. PpgL, a periplasmic gluconolactonase, has been reported to be involved in P. aeruginosa quorum-sensing (QS) system regulation. However, the detailed function and catalysis mechanism remain elusive. Here, the crystal structure of PpgL is described in the current study, along with biochemical analysis, revealing that PpgL is a typical beta-propeller enzyme with unique metal-independent lactone hydrolysis activity. Consequently, comparative analysis of seven-bladed propeller lactone-catalyzing enzymes and mutagenesis studies identify the critical sites which contribute to the diverse catalytic and substrate recognition functions. In addition, the reduced biofilm formation and attenuated invasion phenotype resulting from deletion of ppgL confirm the importance of PpgL in P. aeruginosa pathogenesis. These results suggest that PpgL is a potential target for developing new agents against the diseases caused by P. aeruginosa.

Structural and Functional Insights into PpgL, a Metal-Independent beta-Propeller Gluconolactonase That Contributes to Pseudomonas aeruginosa Virulence.,Song YJ, Wang KL, Shen YL, Gao J, Li T, Zhu YB, Li CC, He LH, Zhou QX, Zhao NL, Zhao C, Yang J, Huang Q, Mu XY, Li H, Dou DF, Liu C, He JH, Sun B, Bao R Infect Immun. 2019 Mar 25;87(4). pii: IAI.00847-18. doi: 10.1128/IAI.00847-18., Print 2019 Apr. PMID:30642898^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.