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6jyg
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of L-threonine dehydrogenase from Phytophthora infestans== | |
| + | <StructureSection load='6jyg' size='340' side='right'caption='[[6jyg]], [[Resolution|resolution]] 2.31Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6jyg]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Phytophthora_infestans_T30-4 Phytophthora infestans T30-4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JYG FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=PE8:3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL'>PE8</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jyg OCA], [https://pdbe.org/6jyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jyg RCSB], [https://www.ebi.ac.uk/pdbsum/6jyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jyg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D0N3N0_PHYIT D0N3N0_PHYIT] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We report, for the first time, the three-dimensional structure and biochemical properties of a UDP-galactose 4-epimerase-like l-threonine 3-dehydrogenase (GalE-like L-ThrDH) from Phytophthora infestans, a plant disease-causing fungus. We identified GalE-like L-ThrDH using Kyoto Encyclopedia of Genes and Genomes (KEGG) database as a candidate target for the development of a new fungicide. The GalE-like L-ThrDH gene was expressed in Escherichia coli, and its product was purified and characterized. N-Acetylglycine was found to act as a competitive inhibitor of the enzyme (Ki =0.18 mM). The crystal structure of the unique hexameric GalE-like L-ThrDH was determined using the molecular replacement method at a resolution of 2.3 A, in the presence of NAD(+) and citrate, an analogue of the substrate. Based on the molecular docking simulation, N-acetylglycine molecule was modeled into the active site and the binding mode and inhibition mechanism of N-acetylglycine were elucidated. | ||
| - | + | Catalytic properties and crystal structure of UDP-galactose 4-epimerase-like l-threonine 3-dehydrogenase from Phytophthora infestans.,Yoneda K, Nagano R, Mikami T, Sakuraba H, Fukui K, Araki T, Ohshima T Enzyme Microb Technol. 2020 Oct;140:109627. doi: 10.1016/j.enzmictec.2020.109627., Epub 2020 Jun 24. PMID:32912687<ref>PMID:32912687</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6jyg" style="background-color:#fffaf0;"></div> |
| - | [[Category: Sakuraba | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Phytophthora infestans T30-4]] | ||
| + | [[Category: Ohshima T]] | ||
| + | [[Category: Sakuraba H]] | ||
| + | [[Category: Yoneda K]] | ||
Current revision
Crystal Structure of L-threonine dehydrogenase from Phytophthora infestans
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