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| | <StructureSection load='6lnm' size='340' side='right'caption='[[6lnm]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='6lnm' size='340' side='right'caption='[[6lnm]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6lnm]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LNM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LNM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lnm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LNM FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cask ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat]), Apba1, Mint1, X11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lnm OCA], [https://pdbe.org/6lnm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lnm RCSB], [https://www.ebi.ac.uk/pdbsum/6lnm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lnm ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lnm OCA], [http://pdbe.org/6lnm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lnm RCSB], [http://www.ebi.ac.uk/pdbsum/6lnm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lnm ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CSKP_RAT CSKP_RAT]] Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins, and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.<ref>PMID:10749215</ref> [[http://www.uniprot.org/uniprot/APBA1_MOUSE APBA1_MOUSE]] Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the amyloid-beta precursor protein (APP) and hence formation of AAP-beta (By similarity). | + | [https://www.uniprot.org/uniprot/CSKP_RAT CSKP_RAT] Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins, and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.<ref>PMID:10749215</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Non-specific serine/threonine protein kinase]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Cai, Q]] | + | [[Category: Cai Q]] |
| - | [[Category: Zhang, M]] | + | [[Category: Zhang M]] |
| - | [[Category: Calmodulin-dependent protein kinase]]
| + | |
| - | [[Category: Camk]]
| + | |
| - | [[Category: Cask]]
| + | |
| - | [[Category: Lin-2]]
| + | |
| - | [[Category: Maguk]]
| + | |
| - | [[Category: Mint1]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
CSKP_RAT Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins, and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.[1]
Publication Abstract from PubMed
CASK forms an evolutionarily conserved tripartite complex with Mint1 and Veli critical for neuronal synaptic transmission and cell polarity. The CASK CaM kinase (CaMK) domain, in addition to interacting with Mint1, can also bind to many different target proteins, although the mechanism governing CASK-CaMK/target interaction selectivity is unclear. Here, we demonstrate that an extended sequence in the N-terminal unstructured region of Mint1 binds to CASK-CaMK with a dissociation constant of approximately 7.5 nM. The high-resolution crystal structure of CASK-CaMK in complex with this Mint1 fragment reveals that the C-lobe of CASK-CaMK binds to a short sequence common to known CaMK targets and the N-lobe of CaMK engages an alpha helix that is unique to Mint1. Biochemical experiments together with structural analysis reveal that the CASK and Mint1 interaction is not regulated by Ca(2+)/CaM. The CASK/Mint1 complex structure provides mechanistic explanations for several CASK mutations identified in patients with brain disorders and cancers.
Structural Basis for the High-Affinity Interaction between CASK and Mint1.,Wu X, Cai Q, Chen Y, Zhu S, Mi J, Wang J, Zhang M Structure. 2020 Jun 2;28(6):664-673.e3. doi: 10.1016/j.str.2020.04.001. Epub 2020, Apr 28. PMID:32348748[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hsueh YP, Wang TF, Yang FC, Sheng M. Nuclear translocation and transcription regulation by the membrane-associated guanylate kinase CASK/LIN-2. Nature. 2000 Mar 16;404(6775):298-302. PMID:10749215 doi:http://dx.doi.org/10.1038/35005118
- ↑ Wu X, Cai Q, Chen Y, Zhu S, Mi J, Wang J, Zhang M. Structural Basis for the High-Affinity Interaction between CASK and Mint1. Structure. 2020 Jun 2;28(6):664-673.e3. doi: 10.1016/j.str.2020.04.001. Epub 2020, Apr 28. PMID:32348748 doi:http://dx.doi.org/10.1016/j.str.2020.04.001
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