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| | ==G PROTEIN-BOUND CONFORMATION OF MASTOPARAN-X, NMR, 14 STRUCTURES== | | ==G PROTEIN-BOUND CONFORMATION OF MASTOPARAN-X, NMR, 14 STRUCTURES== |
| - | <StructureSection load='1a13' size='340' side='right'caption='[[1a13]], [[NMR_Ensembles_of_Models | 14 NMR models]]' scene=''> | + | <StructureSection load='1a13' size='340' side='right'caption='[[1a13]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1a13]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Japanese_hornet Japanese hornet]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A13 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1a13]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vespa_simillima_xanthoptera Vespa simillima xanthoptera]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A13 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a13 OCA], [https://pdbe.org/1a13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a13 RCSB], [https://www.ebi.ac.uk/pdbsum/1a13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a13 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a13 OCA], [https://pdbe.org/1a13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a13 RCSB], [https://www.ebi.ac.uk/pdbsum/1a13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a13 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/MAST_VESXA MAST_VESXA]] Mast cell degranulating peptide. Activates G proteins that couple to phospholipase C.
| + | [https://www.uniprot.org/uniprot/MAST_VESXA MAST_VESXA] Mast cell degranulating peptide. Activates G proteins that couple to phospholipase C. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Japanese hornet]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kohno, T]] | + | [[Category: Vespa simillima xanthoptera]] |
| - | [[Category: Kusunoki, H]] | + | [[Category: Kohno T]] |
| - | [[Category: Miyazawa, T]] | + | [[Category: Kusunoki H]] |
| - | [[Category: Sato, K]] | + | [[Category: Miyazawa T]] |
| - | [[Category: Wakamatsu, K]] | + | [[Category: Sato K]] |
| - | [[Category: Mast cell degranulation]]
| + | [[Category: Wakamatsu K]] |
| Structural highlights
Function
MAST_VESXA Mast cell degranulating peptide. Activates G proteins that couple to phospholipase C.
Publication Abstract from PubMed
Mastoparans, a family of tetradecapeptides from wasp venom, have been used as convenient low molecular weight models of receptors coupled to GTP-binding regulatory proteins (G proteins) for the understanding of the interaction between G proteins and receptors. Sukumar and Higashijima have analyzed the conformation of mastoparan-X (MP-X) bound to the G protein alpha-subunit using proton two-dimensional transferred nuclear Overhauser effect (TRNOE) spectroscopy [Sukumar, M., and Higashijima, T. (1992) J. Biol. Chem., 267, 21421-21424]. The resultant structure, however, was not well-defined due to severe overlap of peptide proton resonances. To determine the G protein-bound conformation of MP-X in detail, we have analyzed this interaction by heteronuclear multidimensional TRNOE experiments of MP-X uniformly enriched with 15N and/or 13C. By solving the overlap problem, we were able to determine the precise conformation of MP-X bound to Gi1alpha: the peptide adopts an amphiphilic alpha-helix from Trp3 to C-terminal Leu14, and the atomic root-mean-square deviation (rmsd) values in this portion about the averaged coordinates were 0.27 +/- 0.07 A for the backbone atoms (N, Calpha, C') and 0.84 +/- 0.16 A for all heavy atoms. These values are much smaller than the corresponding rmsd values of the structures obtained from the proton 2D TRNOE spectrum alone: 1.70 +/- 0.41 A for the backbone atoms (N, Calpha, C') and 2.84 +/- 0.51 A for all heavy atoms. Our results indicate that the heteronuclear multidimensional TRNOE experiments of peptides uniformly enriched with stable isotopes are a very powerful tool for analyzing the conformation of short peptides bound to large proteins. We will also discuss the structure-activity relationships of mastoparans in activating G proteins on the basis of the precise structure of MP-X bound to Gi1alpha.
G protein-bound conformation of mastoparan-X: heteronuclear multidimensional transferred nuclear overhauser effect analysis of peptide uniformly enriched with 13C and 15N.,Kusunoki H, Wakamatsu K, Sato K, Miyazawa T, Kohno T Biochemistry. 1998 Apr 7;37(14):4782-90. PMID:9537994[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kusunoki H, Wakamatsu K, Sato K, Miyazawa T, Kohno T. G protein-bound conformation of mastoparan-X: heteronuclear multidimensional transferred nuclear overhauser effect analysis of peptide uniformly enriched with 13C and 15N. Biochemistry. 1998 Apr 7;37(14):4782-90. PMID:9537994 doi:10.1021/bi972756p
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