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1d0w

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(New page: 200px<br /><applet load="1d0w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d0w" /> '''SOLUTION STRUCTURE OF LACTAM-BRIDGED C-TERMI...)
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[[Image:1d0w.jpg|left|200px]]<br /><applet load="1d0w" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1d0w" />
 
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'''SOLUTION STRUCTURE OF LACTAM-BRIDGED C-TERMINAL ANALOGUE-I OF NEUROPEPTIDE Y'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF LACTAM-BRIDGED C-TERMINAL ANALOGUE-I OF NEUROPEPTIDE Y==
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The importance of helical structure in an analogue of NPY selective for, the Y2 receptor, Ac[Leu28,31]NPY24-36, has been investigated by, introducing a lactam bridge between positions 28 and 32. The resulting, analogue, Ac-cyclo28/32[Ala24,Lys28,Leu31,Glu32]NPY24-36, is a potent, Y2-selective agonist. Structural analysis by NMR shows that this analogue, forms a helical structure in a 40% trifluoroethanol/water mixture, whereas, in water only the region around the lactam bridge (Lys28-Glu32) adopts, helical-like structure, with both N- and C-termini being poorly defined., The observation of well-defined helical structure in aqueous TFE contrasts, with that reported for a similar analogue, Ac-cyclo28/32[Lys28,Glu32]NPY25-36 (Rist et al. FEBS Lett. 1996, 394, 169-173), which consisted of a hairpin-like structure that brought the N-, and C-termini into proximity. We have therefore determined the structures, of this analogue, as well as those of, Ac-cyclo28/32[Ala24,Lys28,Leu31,Glu32]NPY24-36 and, Ac-cyclo28/32[Ala24,Lys28,Glu32]NPY24-36, under identical solution, conditions (30% TFE/H2O mixture at 308 K) and find essentially the same, helical structure in all three peptides. These findings support the, proposal that these Y2-selective analogues adopt a helical structure when, bound to the Y2 receptor.
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<StructureSection load='1d0w' size='340' side='right'caption='[[1d0w]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1d0w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D0W FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AYA:N-ACETYLALANINE'>AYA</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0w OCA], [https://pdbe.org/1d0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d0w RCSB], [https://www.ebi.ac.uk/pdbsum/1d0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d0w ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The importance of helical structure in an analogue of NPY selective for the Y2 receptor, Ac[Leu28,31]NPY24-36, has been investigated by introducing a lactam bridge between positions 28 and 32. The resulting analogue, Ac-cyclo28/32[Ala24,Lys28,Leu31,Glu32]NPY24-36, is a potent Y2-selective agonist. Structural analysis by NMR shows that this analogue forms a helical structure in a 40% trifluoroethanol/water mixture, whereas in water only the region around the lactam bridge (Lys28-Glu32) adopts helical-like structure, with both N- and C-termini being poorly defined. The observation of well-defined helical structure in aqueous TFE contrasts with that reported for a similar analogue, Ac-cyclo28/32[Lys28,Glu32]NPY25-36 (Rist et al. FEBS Lett. 1996, 394, 169-173), which consisted of a hairpin-like structure that brought the N- and C-termini into proximity. We have therefore determined the structures of this analogue, as well as those of Ac-cyclo28/32[Ala24,Lys28,Leu31,Glu32]NPY24-36 and Ac-cyclo28/32[Ala24,Lys28,Glu32]NPY24-36, under identical solution conditions (30% TFE/H2O mixture at 308 K) and find essentially the same helical structure in all three peptides. These findings support the proposal that these Y2-selective analogues adopt a helical structure when bound to the Y2 receptor.
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==About this Structure==
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Stabilization of the helical structure of Y2-selective analogues of neuropeptide Y by lactam bridges.,Yao S, Smith-White MA, Potter EK, Norton RS J Med Chem. 2002 May 23;45(11):2310-8. PMID:12014969<ref>PMID:12014969</ref>
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1D0W is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D0W OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Stabilization of the helical structure of Y2-selective analogues of neuropeptide Y by lactam bridges., Yao S, Smith-White MA, Potter EK, Norton RS, J Med Chem. 2002 May 23;45(11):2310-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12014969 12014969]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1d0w" style="background-color:#fffaf0;"></div>
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[[Category: Norton, R.S.]]
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== References ==
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[[Category: Yao, S.]]
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<references/>
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[[Category: NH2]]
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__TOC__
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[[Category: helix]]
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</StructureSection>
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[[Category: lactam-bridged]]
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[[Category: Large Structures]]
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[[Category: Norton RS]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:11:26 2007''
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[[Category: Yao S]]

Current revision

SOLUTION STRUCTURE OF LACTAM-BRIDGED C-TERMINAL ANALOGUE-I OF NEUROPEPTIDE Y

PDB ID 1d0w

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