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1dg2
From Proteopedia
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| - | [[Image:1dg2.gif|left|200px]] | ||
| - | + | ==SOLUTION CONFORMATION OF A-CONOTOXIN AUIB== | |
| - | + | <StructureSection load='1dg2' size='340' side='right'caption='[[1dg2]]' scene=''> | |
| - | | | + | == Structural highlights == |
| - | | | + | <table><tr><td colspan='2'>[[1dg2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_aulicus Conus aulicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DG2 FirstGlance]. <br> |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dg2 OCA], [https://pdbe.org/1dg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dg2 RCSB], [https://www.ebi.ac.uk/pdbsum/1dg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dg2 ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | ''' | + | == Function == |
| - | + | [https://www.uniprot.org/uniprot/CA1B_CONAL CA1B_CONAL] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks mammalian nAChR alpha-3/beta-4 subunits.<ref>PMID:9786965</ref> | |
| - | + | <div style="background-color:#fffaf0;"> | |
| - | == | + | == Publication Abstract from PubMed == |
The neuronal nicotinic acetylcholine receptors constitute a highly diverse group, with subtypes consisting of pentameric combinations of alpha and beta subunits. alpha-Conotoxins are a homologous series of small peptides that antagonize these receptors. We present the three-dimensional solution structure of alpha-conotoxin AuIB, the first 15-residue alpha-conotoxin known to selectively block the alpha(3)beta(4) nicotinic acetylcholine receptor subtype. The pairwise backbone and heavy-atom root mean square deviation for an ensemble of 20 structures are 0.269 and 0.720 A, respectively. The overall fold of alpha-conotoxin AuIB closely resembles that of the alpha4/7 subfamily alpha-conotoxins. However, the absence of Tyr(15), normally present in other alpha4/7 members, results in tight bending of the backbone at the C terminus and effectively renders Asp(14) to assume the spatial location of Tyr(15) present in other neuronal alpha4/7 alpha-conotoxins. Structural comparison of alpha-conotoxin AuIB with the alpha(3)beta(2) subtype-specific alpha-conotoxin MII shows different electrostatic surface charge distributions, which may be important in differential receptor subtype recognition. | The neuronal nicotinic acetylcholine receptors constitute a highly diverse group, with subtypes consisting of pentameric combinations of alpha and beta subunits. alpha-Conotoxins are a homologous series of small peptides that antagonize these receptors. We present the three-dimensional solution structure of alpha-conotoxin AuIB, the first 15-residue alpha-conotoxin known to selectively block the alpha(3)beta(4) nicotinic acetylcholine receptor subtype. The pairwise backbone and heavy-atom root mean square deviation for an ensemble of 20 structures are 0.269 and 0.720 A, respectively. The overall fold of alpha-conotoxin AuIB closely resembles that of the alpha4/7 subfamily alpha-conotoxins. However, the absence of Tyr(15), normally present in other alpha4/7 members, results in tight bending of the backbone at the C terminus and effectively renders Asp(14) to assume the spatial location of Tyr(15) present in other neuronal alpha4/7 alpha-conotoxins. Structural comparison of alpha-conotoxin AuIB with the alpha(3)beta(2) subtype-specific alpha-conotoxin MII shows different electrostatic surface charge distributions, which may be important in differential receptor subtype recognition. | ||
| - | + | Nuclear magnetic resonance solution conformation of alpha-conotoxin AuIB, an alpha(3)beta(4) subtype-selective neuronal nicotinic acetylcholine receptor antagonist.,Cho JH, Mok KH, Olivera BM, McIntosh JM, Park KH, Han KH J Biol Chem. 2000 Mar 24;275(12):8680-5. PMID:10722709<ref>PMID:10722709</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 1dg2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Conus aulicus]] | [[Category: Conus aulicus]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Cho | + | [[Category: Cho J-H]] |
| - | [[Category: Han | + | [[Category: Han K-H]] |
| - | [[Category: McIntosh | + | [[Category: McIntosh JM]] |
| - | [[Category: Mok | + | [[Category: Mok KH]] |
| - | [[Category: Olivera | + | [[Category: Olivera BM]] |
| - | [[Category: Park | + | [[Category: Park K-H]] |
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Current revision
SOLUTION CONFORMATION OF A-CONOTOXIN AUIB
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Categories: Conus aulicus | Large Structures | Cho J-H | Han K-H | McIntosh JM | Mok KH | Olivera BM | Park K-H
