1eht

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[[Image:1eht.jpg|left|200px]]
 
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==THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 STRUCTURES==
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The line below this paragraph, containing "STRUCTURE_1eht", creates the "Structure Box" on the page.
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<StructureSection load='1eht' size='340' side='right'caption='[[1eht]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1eht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EHT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TEP:THEOPHYLLINE'>TEP</scene></td></tr>
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{{STRUCTURE_1eht| PDB=1eht | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eht OCA], [https://pdbe.org/1eht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eht RCSB], [https://www.ebi.ac.uk/pdbsum/1eht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eht ProSAT]</span></td></tr>
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</table>
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'''THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 STRUCTURES'''
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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==Overview==
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To visualize the interplay of RNA structural interactions in a ligand binding site, we have determined the solution structure of a high affinity RNA-theophylline complex using NMR spectroscopy. The structure provides insight into the ability of this in vitro selected RNA to discriminate theophylline from the structurally similar molecule caffeine. Numerous RNA structural motifs combine to form a well-ordered binding pocket where an intricate network of hydrogen bonds and stacking interactions lock the theophylline into the complex. Two internal loops interact to form the binding site which consists of a sandwich of three base triples. The complex also contains novel base-zipper and 1-3-2 stacking motifs, in addition to an adenosine platform and a reversed sugar. An important feature of the RNA is that many of the conserved core residues participate in multiple overlapping tertiary interactions. This complex illustrates how interlocking structural motifs can be assembled into a highly specific ligand-binding site that possesses high levels of affinity and molecular discrimination.
To visualize the interplay of RNA structural interactions in a ligand binding site, we have determined the solution structure of a high affinity RNA-theophylline complex using NMR spectroscopy. The structure provides insight into the ability of this in vitro selected RNA to discriminate theophylline from the structurally similar molecule caffeine. Numerous RNA structural motifs combine to form a well-ordered binding pocket where an intricate network of hydrogen bonds and stacking interactions lock the theophylline into the complex. Two internal loops interact to form the binding site which consists of a sandwich of three base triples. The complex also contains novel base-zipper and 1-3-2 stacking motifs, in addition to an adenosine platform and a reversed sugar. An important feature of the RNA is that many of the conserved core residues participate in multiple overlapping tertiary interactions. This complex illustrates how interlocking structural motifs can be assembled into a highly specific ligand-binding site that possesses high levels of affinity and molecular discrimination.
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==About this Structure==
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Interlocking structural motifs mediate molecular discrimination by a theophylline-binding RNA.,Zimmermann GR, Jenison RD, Wick CL, Simorre JP, Pardi A Nat Struct Biol. 1997 Aug;4(8):644-9. PMID:9253414<ref>PMID:9253414</ref>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EHT OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Interlocking structural motifs mediate molecular discrimination by a theophylline-binding RNA., Zimmermann GR, Jenison RD, Wick CL, Simorre JP, Pardi A, Nat Struct Biol. 1997 Aug;4(8):644-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9253414 9253414]
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</div>
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[[Category: Pardi, A.]]
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<div class="pdbe-citations 1eht" style="background-color:#fffaf0;"></div>
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[[Category: Zimmermann, G R.]]
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== References ==
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[[Category: Ribonucleic acid]]
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<references/>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:07:01 2008''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pardi A]]
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[[Category: Zimmermann GR]]

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THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 STRUCTURES

PDB ID 1eht

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