1evo
From Proteopedia
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| - | [[Image:1evo.gif|left|200px]]<br /><applet load="1evo" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1evo" /> | ||
| - | '''NMR OBSERVATION OF A NOVEL C-TETRAD'''<br /> | ||
| - | == | + | ==NMR OBSERVATION OF A NOVEL C-TETRAD== |
| + | <StructureSection load='1evo' size='340' side='right'caption='[[1evo]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1evo]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EVO FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1evo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1evo OCA], [https://pdbe.org/1evo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1evo RCSB], [https://www.ebi.ac.uk/pdbsum/1evo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1evo ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
We report the NMR structure of the DNA sequence d-TGGGCGGT in Na(+) solutions at neutral pH, containing a repeat sequence from SV40 viral genome. The structure is a novel quadruplex incorporating the C-tetrad formed by symmetrical pairing of four Cs via NH(2)&bond;O(2) H-bonds in a plane. The C-tetrad has a wider cavity compared to G-tetrads and stacks well over the adjacent G4-tetrad, but poorly on the G6 tetrad. The quadruplex helix is largely underwound by 8-10 degrees compared to B-DNA except at the C5-G6 step. To our knowledge this is the first report of C-tetrad formation in DNA structures, and would be of significance from the point of view of both structural diversity and specific recognition. | We report the NMR structure of the DNA sequence d-TGGGCGGT in Na(+) solutions at neutral pH, containing a repeat sequence from SV40 viral genome. The structure is a novel quadruplex incorporating the C-tetrad formed by symmetrical pairing of four Cs via NH(2)&bond;O(2) H-bonds in a plane. The C-tetrad has a wider cavity compared to G-tetrads and stacks well over the adjacent G4-tetrad, but poorly on the G6 tetrad. The quadruplex helix is largely underwound by 8-10 degrees compared to B-DNA except at the C5-G6 step. To our knowledge this is the first report of C-tetrad formation in DNA structures, and would be of significance from the point of view of both structural diversity and specific recognition. | ||
| - | + | NMR observation of a novel C-tetrad in the structure of the SV40 repeat sequence GGGCGG.,Patel PK, Bhavesh NS, Hosur RV Biochem Biophys Res Commun. 2000 Apr 21;270(3):967-71. PMID:10772934<ref>PMID:10772934</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 1evo" style="background-color:#fffaf0;"></div> |
| - | [[Category: Bhavesh | + | == References == |
| - | [[Category: Hosur | + | <references/> |
| - | [[Category: Patel | + | __TOC__ |
| - | + | </StructureSection> | |
| - | + | [[Category: Large Structures]] | |
| - | + | [[Category: Bhavesh NS]] | |
| - | + | [[Category: Hosur RV]] | |
| + | [[Category: Patel PK]] | ||
Current revision
NMR OBSERVATION OF A NOVEL C-TETRAD
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