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1eyo

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(New page: 200px<br /><applet load="1eyo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eyo" /> '''SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM C...)
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[[Image:1eyo.jpg|left|200px]]<br /><applet load="1eyo" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eyo" />
 
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'''SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM CONUS TULIPA'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM CONUS TULIPA==
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The three-dimensional solution structure of conotoxin TVIIA, a 30-residue, polypeptide from the venom of the piscivorous cone snail Conus tulipa, has, been determined using 2D 1H NMR spectroscopy. TVIIA contains six cysteine, residues which form a 'four-loop' structural framework common to many, peptides from Conus venoms including the omega-, delta-, kappa-, and, muO-conotoxins. However, TVIIA does not belong to these well-characterized, pharmacological classes of conotoxins, but displays high sequence identity, with conotoxin GS, a muscle sodium channel blocker from Conus geographus., Structure calculations were based on 562 interproton distance restraints, inferred from NOE data, together with 18 backbone and nine side-chain, torsion angle restraints derived from spin-spin coupling constants. The, final family of 20 structures had mean pairwise rms differences over, residues 2-27 of 0.18+/-0.05 A for the backbone atoms and 1.39+/-0.33 A, for all heavy atoms. The structure consists of a triple-stranded, antiparallel beta sheet with +2x, -1 topology (residues 7-9, 16-20 and, 23-27) and several beta turns. The core of the molecule is formed by three, disulfide bonds which form a cystine knot motif common to many toxic and, inhibitory polypeptides. The global fold, molecular shape and distribution, of amino-acid sidechains in TVIIA is similar to that previously reported, for conotoxin GS, and comparison with other four-loop conotoxin structures, provides further indication that TVIIA and GS represent a new and distinct, subgroup of this structural family. The structure of TVIIA determined in, this study provides the basis for determining a structure-activity, relationship for these molecules and their interaction with target, receptors.
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<StructureSection load='1eyo' size='340' side='right'caption='[[1eyo]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1eyo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_tulipa Conus tulipa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EYO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyo OCA], [https://pdbe.org/1eyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eyo RCSB], [https://www.ebi.ac.uk/pdbsum/1eyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eyo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/U7A_CONTU U7A_CONTU] By structural similarity with conotoxin GS, may inhibit the sodium channel (Nav). No effect was observed upon intracranial injections into mice and intraperitoneal injections into goldfish (25 ug).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three-dimensional solution structure of conotoxin TVIIA, a 30-residue polypeptide from the venom of the piscivorous cone snail Conus tulipa, has been determined using 2D 1H NMR spectroscopy. TVIIA contains six cysteine residues which form a 'four-loop' structural framework common to many peptides from Conus venoms including the omega-, delta-, kappa-, and muO-conotoxins. However, TVIIA does not belong to these well-characterized pharmacological classes of conotoxins, but displays high sequence identity with conotoxin GS, a muscle sodium channel blocker from Conus geographus. Structure calculations were based on 562 interproton distance restraints inferred from NOE data, together with 18 backbone and nine side-chain torsion angle restraints derived from spin-spin coupling constants. The final family of 20 structures had mean pairwise rms differences over residues 2-27 of 0.18+/-0.05 A for the backbone atoms and 1.39+/-0.33 A for all heavy atoms. The structure consists of a triple-stranded, antiparallel beta sheet with +2x, -1 topology (residues 7-9, 16-20 and 23-27) and several beta turns. The core of the molecule is formed by three disulfide bonds which form a cystine knot motif common to many toxic and inhibitory polypeptides. The global fold, molecular shape and distribution of amino-acid sidechains in TVIIA is similar to that previously reported for conotoxin GS, and comparison with other four-loop conotoxin structures provides further indication that TVIIA and GS represent a new and distinct subgroup of this structural family. The structure of TVIIA determined in this study provides the basis for determining a structure-activity relationship for these molecules and their interaction with target receptors.
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==About this Structure==
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Conotoxin TVIIA, a novel peptide from the venom of Conus tulipa 2. Three-dimensional solution structure.,Hill JM, Alewood PF, Craik DJ Eur J Biochem. 2000 Aug;267(15):4649-57. PMID:10903497<ref>PMID:10903497</ref>
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1EYO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EYO OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Conotoxin TVIIA, a novel peptide from the venom of Conus tulipa 2. Three-dimensional solution structure., Hill JM, Alewood PF, Craik DJ, Eur J Biochem. 2000 Aug;267(15):4649-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10903497 10903497]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1eyo" style="background-color:#fffaf0;"></div>
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[[Category: Alewood, P.F.]]
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== References ==
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[[Category: Craik, D.J.]]
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<references/>
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[[Category: Hill, J.M.]]
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__TOC__
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[[Category: cystine knot motif]]
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</StructureSection>
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[[Category: Conus tulipa]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:26:20 2007''
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[[Category: Large Structures]]
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[[Category: Alewood PF]]
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[[Category: Craik DJ]]
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[[Category: Hill JM]]

Current revision

SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM CONUS TULIPA

PDB ID 1eyo

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