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1f3s

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(New page: 200px<br /><applet load="1f3s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f3s" /> '''SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG...)
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[[Image:1f3s.gif|left|200px]]<br /><applet load="1f3s" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f3s" />
 
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'''SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.'''<br />
 
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==Overview==
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==Solution Structure of DNA Sequence GGGTTCAGG Forms GGGG Tetrade and G(C-A) Triad.==
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We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to, form a 2-fold symmetric G-quadruplex in which G(syn)., G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A), triads. The NMR-based solution structural analysis was greatly aided by, monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen, bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled, sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure, establishes that the guanine base-pairs with the cytosine through, Watson-Crick G.C pair formation and with adenine through sheared G.A, mismatch formation within the G.(C-A) triad. A model of triad DNA was, constructed that contains the experimentally determined G.(C-A) triad, alignment as the repeating stacked unit.
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<StructureSection load='1f3s' size='340' side='right'caption='[[1f3s]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f3s]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F3S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f3s OCA], [https://pdbe.org/1f3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f3s RCSB], [https://www.ebi.ac.uk/pdbsum/1f3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f3s ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to form a 2-fold symmetric G-quadruplex in which G(syn). G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A) triads. The NMR-based solution structural analysis was greatly aided by monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure establishes that the guanine base-pairs with the cytosine through Watson-Crick G.C pair formation and with adenine through sheared G.A mismatch formation within the G.(C-A) triad. A model of triad DNA was constructed that contains the experimentally determined G.(C-A) triad alignment as the repeating stacked unit.
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==About this Structure==
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A two-stranded template-based approach to G.(C-A) triad formation: designing novel structural elements into an existing DNA framework.,Kettani A, Basu G, Gorin A, Majumdar A, Skripkin E, Patel DJ J Mol Biol. 2000 Aug 4;301(1):129-46. PMID:10926497<ref>PMID:10926497</ref>
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1F3S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A two-stranded template-based approach to G.(C-A) triad formation: designing novel structural elements into an existing DNA framework., Kettani A, Basu G, Gorin A, Majumdar A, Skripkin E, Patel DJ, J Mol Biol. 2000 Aug 4;301(1):129-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10926497 10926497]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1f3s" style="background-color:#fffaf0;"></div>
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[[Category: Basu, G.]]
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== References ==
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[[Category: Gorin, A.]]
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<references/>
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[[Category: Kettani, A.]]
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__TOC__
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[[Category: Majumdar, A.]]
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</StructureSection>
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[[Category: Patel, D.J.]]
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[[Category: Large Structures]]
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[[Category: Skripkin, E.]]
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[[Category: Basu G]]
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[[Category: 15n-labeled dna]]
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[[Category: Gorin A]]
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[[Category: g-quadruplex]]
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[[Category: Kettani A]]
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[[Category: g.(c-a) base triad]]
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[[Category: Majumdar A]]
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[[Category: triad dna model]]
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[[Category: Patel DJ]]
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[[Category: uniform 13c]]
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[[Category: Skripkin E]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:14:30 2007''
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Current revision

Solution Structure of DNA Sequence GGGTTCAGG Forms GGGG Tetrade and G(C-A) Triad.

PDB ID 1f3s

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