This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
6m5m
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6m5m is ON HOLD Authors: Description: Category: Unreleased Structures) |
|||
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==SPL-1 - GlcNAc complex== | |
| + | <StructureSection load='6m5m' size='340' side='right'caption='[[6m5m]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6m5m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saxidomus_purpurata Saxidomus purpurata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M5M FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m5m OCA], [https://pdbe.org/6m5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m5m RCSB], [https://www.ebi.ac.uk/pdbsum/6m5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m5m ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A2Z6G7U6_9BIVA A0A2Z6G7U6_9BIVA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The C-type lectins SPL-1 and SPL-2 from the bivalve Saxidomus purpuratus are composed of A and B chains and of two B chains, respectively. They bind specific carbohydrates containing acetamido groups, such as N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc), in a Ca(2+)-independent manner. Unlike ordinary C-type lectins, which require Ca(2+) ions for carbohydrate recognition, these lectins recognize specific carbohydrates mainly through interactions with the acetamido group without Ca(2+) ions, even though Ca(2+) enhances the binding affinity of these lectins, especially SPL-1. In the present study, the crystal structure of the SPL-1-GlcNAc complex in the presence of Ca(2+) revealed that the binding of SPL-1 to GlcNAc is stabilized by hydrogen bonds to the water molecule(s) coordinating Ca(2+), whereas in ordinary C-type lectins Ca(2+) directly forms coordinate bonds to the hydroxy groups of carbohydrates. These differences may also allow SPL-1 and SPL-2 to recognize both GlcNAc and GalNAc, which have different orientations of the 4-hydroxy group. | ||
| - | + | Novel carbohydrate-recognition mode of the invertebrate C-type lectin SPL-1 from Saxidomus purpuratus revealed by the GlcNAc-complex crystal in the presence of Ca(2).,Unno H, Higuchi S, Goda S, Hatakeyama T Acta Crystallogr F Struct Biol Commun. 2020 Jun 1;76(Pt 6):271-277. doi:, 10.1107/S2053230X20007256. Epub 2020 Jun 5. PMID:32510468<ref>PMID:32510468</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6m5m" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saxidomus purpurata]] | ||
| + | [[Category: Hatakeyama T]] | ||
| + | [[Category: Unno H]] | ||
Current revision
SPL-1 - GlcNAc complex
| |||||||||||
