This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7c7d
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7c7d is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures) |
|||
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3== | |
| + | <StructureSection load='7c7d' size='340' side='right'caption='[[7c7d]], [[Resolution|resolution]] 1.16Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7c7d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_thermodiastaticus Streptomyces thermodiastaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C7D FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.16Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c7d OCA], [https://pdbe.org/7c7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c7d RCSB], [https://www.ebi.ac.uk/pdbsum/7c7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c7d ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A7G1KW31_9ACTN A0A7G1KW31_9ACTN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | alpha-1,3-Glucan is a homopolymer composed of D-glucose (Glc) and it is an extracellular polysaccharide found in dental plaque due to Streptococcus species. alpha-1,3-Glucanase from Streptomyces thermodiastaticus strain HF3-3 (Agl-ST) has been identified as a thermostable alpha-1,3-glucanase, which is classified into glycoside hydrolase family 87 (GH87) and specifically hydrolyzes alpha-1,3-glucan with an endo-action. The enzyme has a potential to inhibit the production of dental plaque and to be used for biotechnological applications. Here we show the structure of the catalytic unit of Agl-ST determined at 1.16 A resolution using X-ray crystallography. The catalytic unit is composed of two modules, a beta-sandwich fold module, and a right-handed beta-helix fold module, which resembles other structural characterized GH87 enzymes from Bacillus circulans str. KA-304 and Paenibacillus glycanilyticus str. FH11, with moderate sequence identities between each other (approximately 27% between the catalytic units). However, Agl-ST is smaller in size and more thermally stable than the others. A disulfide bond that anchors the C-terminal coil of the beta-helix fold, which is expected to contribute to thermal stability only exists in the catalytic unit of Agl-ST. | ||
| - | + | Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3.,Itoh T, Panti N, Hayashi J, Toyotake Y, Matsui D, Yano S, Wakayama M, Hibi T Biochem Biophys Res Commun. 2020 Oct 8. pii: S0006-291X(20)31894-5. doi:, 10.1016/j.bbrc.2020.09.133. PMID:33041007<ref>PMID:33041007</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7c7d" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces thermodiastaticus]] | ||
| + | [[Category: Hayashi J]] | ||
| + | [[Category: Hibi T]] | ||
| + | [[Category: Itoh T]] | ||
| + | [[Category: Panti N]] | ||
| + | [[Category: Suyotha W]] | ||
| + | [[Category: Toyotake Y]] | ||
| + | [[Category: Wakayama M]] | ||
| + | [[Category: Yano S]] | ||
Current revision
Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3
| |||||||||||
