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7cim

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(New page: '''Unreleased structure''' The entry 7cim is ON HOLD until Paper Publication Authors: Okawa, A., Shiba, T., Hayashi, M., Onoue, Y., Murota, M., Sato, D., Inagaki, J., Tamura, T., Harada...)
Current revision (16:08, 29 November 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 7cim is ON HOLD until Paper Publication
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==Crystal structure of L-methionine decarboxylase from Streptomyces sp.590 in complexed with 3-methlythiopropylamine (geminal diamine form).==
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<StructureSection load='7cim' size='340' side='right'caption='[[7cim]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7cim]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._590_KI-2014 Streptomyces sp. 590 KI-2014]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CIM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G0F:[6-methyl-4-[(3-methylsulfanylpropylamino)methyl]-5-oxidanyl-pyridin-3-yl]methyl+dihydrogen+phosphate'>G0F</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cim OCA], [https://pdbe.org/7cim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cim RCSB], [https://www.ebi.ac.uk/pdbsum/7cim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cim ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A0G4DBU7_9ACTN A0A0G4DBU7_9ACTN]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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L-Methionine decarboxylase (MetDC) from Streptomyces sp. 590 is a vitamin B6 -dependent enzyme and catalyzes the non-oxidative decarboxylation of L-methionine to produce 3-methylthiopropylamine and carbon dioxide. We present here the crystal structures of the ligand-free form of MetDC and of several enzymatic reaction intermediates. Group II amino acid decarboxylases have many residues in common around the active site but the residues surrounding the side chain of the substrate differ. Based on information obtained from the crystal structure, and mutational and biochemical experiments, we propose a key role for Gln64 in determining the substrate specificity of MetDC, and for Tyr421 as the acid catalyst that participates in protonation after the decarboxylation reaction.
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Authors: Okawa, A., Shiba, T., Hayashi, M., Onoue, Y., Murota, M., Sato, D., Inagaki, J., Tamura, T., Harada, S., Inagaki, K.
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Structural basis for substrate specificity of L-methionine decarboxylase.,Okawa A, Shiba T, Hayashi M, Onoue Y, Murota M, Sato D, Inagaki J, Tamura T, Harada S, Inagaki K Protein Sci. 2021 Jan 15. doi: 10.1002/pro.4027. PMID:33452696<ref>PMID:33452696</ref>
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Description: Crystal structure of L-methionine decarboxylase from Streptomyces sp.590 in complexed with 3-methlythiopropylamine (geminal diamine form).
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Onoue, Y]]
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<div class="pdbe-citations 7cim" style="background-color:#fffaf0;"></div>
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[[Category: Sato, D]]
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== References ==
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[[Category: Shiba, T]]
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<references/>
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[[Category: Okawa, A]]
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__TOC__
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[[Category: Hayashi, M]]
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</StructureSection>
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[[Category: Murota, M]]
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[[Category: Large Structures]]
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[[Category: Inagaki, J]]
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[[Category: Streptomyces sp. 590 KI-2014]]
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[[Category: Harada, S]]
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[[Category: Harada S]]
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[[Category: Tamura, T]]
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[[Category: Hayashi M]]
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[[Category: Inagaki, K]]
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[[Category: Inagaki J]]
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[[Category: Inagaki K]]
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[[Category: Murota M]]
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[[Category: Okawa A]]
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[[Category: Onoue Y]]
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[[Category: Sato D]]
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[[Category: Shiba T]]
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[[Category: Tamura T]]

Current revision

Crystal structure of L-methionine decarboxylase from Streptomyces sp.590 in complexed with 3-methlythiopropylamine (geminal diamine form).

PDB ID 7cim

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